Dolmatova L S, Romashina V V
Laboratory of Histochemistry, Pacific Oceanological Institute, Vladivostok, Russia.
Eksp Klin Farmakol. 1998 Sep-Oct;61(5):37-41.
The effect of morphine (10(-6) M) and adrenaline (0.2 microgram/10(6) cells) on the oxidant and antioxidant activity in cells of the immunophagocytic system was compared in vitro. Morphine and adrenaline caused different changes in production of active forms of oxygen (AFA) and antioxidant activity of the enzymes superoxide dismutase and glutathione reductase in neutrophils, monocytes, and lymphocytes in human peripheral blood. The effect of these drugs, however, was unidirectional in character in each type of cells. Eleutherococcus (obtained in the drug-store and diluted 10- to 11-fold) or indomethacin (10(-7) M) injected together with morphine removed the morphine-induced increase of APA production in the neutrophils, they also prevented the effect of morphine on enzyme activation in the neutrophils and monocytes, but had no influence on the effect of morphine on the antioxidant enzymes in the neutrophils. The results of the study point to the existence of common mediators in direct action of adrenaline and morphine on the cells of the immunophagocytic system.
在体外比较了吗啡（10⁻⁶ M）和肾上腺素（0.2微克/10⁶个细胞）对免疫吞噬系统细胞中氧化剂和抗氧化剂活性的影响。吗啡和肾上腺素对人外周血中性粒细胞、单核细胞和淋巴细胞中活性氧（AFA）的产生以及超氧化物歧化酶和谷胱甘肽还原酶的抗氧化活性引起了不同的变化。然而，这些药物对每种类型细胞的作用在性质上是单向的。与吗啡一起注射的刺五加（从药店购得并稀释10至11倍）或吲哚美辛（10⁻⁷ M）消除了吗啡诱导的中性粒细胞中APA产生的增加，它们还阻止了吗啡对中性粒细胞和单核细胞中酶激活的作用，但对吗啡对中性粒细胞中抗氧化酶的作用没有影响。该研究结果表明，肾上腺素和吗啡对免疫吞噬系统细胞的直接作用中存在共同的介质。
