Hutton W C, Toribatake Y, Elmer W A, Ganey T M, Tomita K, Whitesides T E
Department of Orthopaedics, Emory University, Atlanta, Georgia.
Spine (Phila Pa 1976). 1998 Dec 1;23(23):2524-37. doi: 10.1097/00007632-199812010-00007.
Coil springs were stretched and attached to produce a compressive force across the lumbar intervertebral discs of dogs for up to 27 weeks.
To test the hypothesis that a high compressive force applied over a period of time affects the production of proteoglycans and collagen by the intervertebral disc cells.
It is a commonly held belief that high forces applied to the intervertebral disc, and to joints in general, play a role in causing degeneration.
Pairs of stainless steel coil springs were stretched and attached to produce a compressive force across the lumbar intervertebral discs (L1-L2 and L3-L4) of 16 dogs. Dogs were killed between 13 and 27 weeks after the springs were attached. The discs (L1-L2 and L3-L4) were excised and assessed using immunohistochemical analyses and enzyme-linked immunosorbent assay; T13-L1 and L4-L5 were used as controls.
The main result relates to a group effect in the six dogs, assessed using enzyme-linked immunosorbent assay, that were generally at the highest values of force for the greatest number of weeks. For the nucleus, but not the anulus, Spearman rank correlations revealed a strong correlation between increases in force and force-weeks (force multiplied by number of weeks) and increases in collagen type I accompanied by decreases in proteoglycans, chondroitin sulfate, and collagen type II for both experimental discs (L1-L2 and L3-L4), as compared with corresponding values in the controls (T13-L1 and L4-L5). In other words, as either the force or the force-weeks increased, the effect on the nucleus became greater.
A high compressive force applied to the disc over a period of time initiates changes in proteoglycans and collagen.
拉伸并连接螺旋弹簧以在犬的腰椎间盘上产生压缩力，持续长达27周。
检验以下假设，即一段时间内施加的高压缩力会影响椎间盘细胞中蛋白聚糖和胶原蛋白的产生。
人们普遍认为，施加于椎间盘以及一般关节的高压力在退变过程中起作用。
拉伸并连接成对的不锈钢螺旋弹簧，以在16只犬的腰椎间盘（L1-L2和L3-L4）上产生压缩力。在连接弹簧后13至27周之间处死犬。切除椎间盘（L1-L2和L3-L4），并使用免疫组织化学分析和酶联免疫吸附测定进行评估；将T13-L1和L4-L5用作对照。
主要结果涉及使用酶联免疫吸附测定评估的6只犬的组效应，这些犬在大多数周内通常处于最高力值。对于髓核而非纤维环，Spearman等级相关性显示，与对照（T13-L1和L4-L5）中的相应值相比，实验椎间盘（L1-L2和L3-L4）的力和力-周数（力乘以周数）增加与I型胶原蛋白增加以及蛋白聚糖、硫酸软骨素和II型胶原蛋白减少之间存在强相关性。换句话说，随着力或力-周数增加，对髓核的影响变得更大。
一段时间内施加于椎间盘的高压缩力会引发蛋白聚糖和胶原蛋白的变化。
