Luft J C, Bengtén E, Clem L W, Miller N W, Wilson M R
Department of Microbiology, University of Mississippi Medical Center, Jackson 39216, USA.
Comp Biochem Physiol B Biochem Mol Biol. 1998 Aug;120(4):675-82. doi: 10.1016/s0305-0491(98)10062-7.
Herein is presented the sequence of a catfish full-length p53 cDNA obtained from a cloned B cell line cDNA library. Southern blot analyses determined that a restriction fragment linked polymorphism (RFLP) existed with PstI among outbred catfish. Western blot analyses demonstrated that, when compared to PBLs, the catfish leukocyte lines express higher levels of p53 protein. Additionally, the results of Western blot analyses and in vitro translation experiments suggest that the catfish leukocyte lines may produce truncated forms of p53 due to internal initiation.
