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Reversible inhibition by pyridoxal 5'-phosphate and irreversible inactivation by urea and guanidine hydrochloride of thymidylate synthase from Lactobacillus leichmannii.

作者信息

Rao K N

机构信息

Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Mumbai, India.

出版信息

Indian J Biochem Biophys. 1998 Aug;35(4):229-35.

PMID:9854903
Abstract

Pyridoxal 5'-phosphate reversibly inhibited thymidylate synthase from Lactobacillus leichmannii. The inhibition was competitive with dUMP (Ki = 1 microM) and non-competitive with 5,10-CH2-THF (Ki = 0.08 microM). Treatment of native or pCMB-treated enzyme with urea (5 M) or guanidine hydrochloride (4 M) resulted in inactivation and dissociation of the homodimer (74 kDa) into monomer (37 kDa).

摘要

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