Madsen J E, Berg-Larsen T, Kirkeby O J, Falch J A, Nordsletten L
Institute for Surgical Research, Rikshospitalet The National Hospital, University of Oslo, Norway.
Acta Orthop Scand. 1998 Oct;69(5):532-6. doi: 10.3109/17453679808997793.
Clodronate was administered daily 28 days before and after an experimental tibial fracture in 35 male rats, and the effect on fracture healing and posttraumatic bone loss was studied. 5 groups were tested. The clodronate/clodronate group received clodronate in daily doses of 10 mg/kg body weight for 28 days before being subjected to a standardized fracture of the right tibia, and during the fracture healing period of 28 days. The clodronate/saline group received clodronate before fracture and saline during the healing period. The saline/clodronate group received saline before and clodronate after fracture. The saline/saline group received saline only, while the control group served as unfractured, untreated controls. After 28 days of fracture healing, the tibias were evaluated with dual energy x-ray absorptiometry, and tested mechanically in a 3-point ventral bending test. Bone mineral content and bone mineral density were approximately 30% higher in the groups receiving clodronate during the experiment, compared to the untreated groups. The weight and cross-sectional area of the fracture callus were equal in all groups. Whether clodronate was administered before the fracture, after the fracture or both, did not affect the bone mineral. Ultimate bending moment, energy absorption, stiffness and deflection were not significantly different between the groups. Our findings suggest that clodronate increases bone mineral both when given before and after a tibial shaft fracture, without affecting fracture healing at 28 days.
在35只雄性大鼠的实验性胫骨骨折前后28天，每天给予氯膦酸盐，并研究其对骨折愈合和创伤后骨质流失的影响。测试了5组。氯膦酸盐/氯膦酸盐组在右侧胫骨进行标准化骨折前28天，每天以10 mg/kg体重的剂量接受氯膦酸盐治疗，并在28天的骨折愈合期内持续给药。氯膦酸盐/生理盐水组在骨折前接受氯膦酸盐治疗，在愈合期接受生理盐水治疗。生理盐水/氯膦酸盐组在骨折前接受生理盐水治疗，在骨折后接受氯膦酸盐治疗。生理盐水/生理盐水组仅接受生理盐水，而对照组为未骨折、未治疗的对照。骨折愈合28天后，用双能X线吸收法评估胫骨，并在三点腹侧弯曲试验中进行力学测试。与未治疗组相比，在实验期间接受氯膦酸盐治疗的组的骨矿物质含量和骨矿物质密度大约高30%。所有组骨折痂的重量和横截面积相等。无论氯膦酸盐是在骨折前、骨折后还是两者都给药，均不影响骨矿物质。各组之间的极限弯矩、能量吸收、刚度和挠度无显著差异。我们的研究结果表明，胫骨骨干骨折前后给予氯膦酸盐均可增加骨矿物质，且在28天时不影响骨折愈合。
