Azeh I, Mäder M, Smirnov A, Beuche W, Nau R, Weber F
Department of Neurology, University of Göttingen, Germany.
Neurosci Lett. 1998 Nov 13;256(3):127-30. doi: 10.1016/s0304-3940(98)00776-9.
Gelatinolytic activity of matrix metalloproteinases (MMPs), particularly MMP-9 and MMP-2, was studied by quantitative zymography in a rabbit model of bacterial meningitis during 24 h after inoculation with Streptococcus pneumoniae. In cerebrospinal fluid (CSF), MMP-2 was constitutively present and its level did not change during the experiment. In contrast, MMP-9, hardly detectable in CSF of healthy animals, increased dramatically. The increase of MMP-9 was correlated with both, an increase of CSF cell count and of total protein concentration. Intrathecal production of MMP-9 and MMP-2 was demonstrated by zymography of equal amounts of total protein from CSF and serum. Homogenates, prepared from various cortical regions of infected rabbits did not show increase of MMP activities. On the other hand, leucocytes isolated from CSF expressed high levels of MMP-9 suggesting a significant contribution of these cells to the elevation of MMP-9 activity in this body fluid.
在肺炎链球菌接种后的24小时内，通过定量酶谱法在兔细菌性脑膜炎模型中研究了基质金属蛋白酶（MMPs），特别是MMP-9和MMP-2的明胶酶活性。在脑脊液（CSF）中，MMP-2持续存在，其水平在实验过程中没有变化。相比之下，在健康动物的脑脊液中几乎检测不到的MMP-9却急剧增加。MMP-9的增加与脑脊液细胞计数和总蛋白浓度的增加均相关。通过对脑脊液和血清中等量总蛋白进行酶谱分析，证实了MMP-9和MMP-2的鞘内产生。从感染兔的各个皮质区域制备的匀浆未显示MMP活性增加。另一方面，从脑脊液中分离出的白细胞表达高水平的MMP-9，表明这些细胞对该体液中MMP-9活性的升高有显著贡献。
