Boyer J D, Chattergoon M, Shah A, Ginsberg R, MacGregor R R, Weiner D B
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
Dev Biol Stand. 1998;95:147-53.
Novel approaches for the generation of more effective vaccines for HIV-1 are of significant importance. In this report we analyse the immunogenicity and efficacy of a DNA vaccination approach in a chimpanzee model system. Three chimpanzees were vaccinated with DNA constructs which express the env, rev, gag and pol proteins. These animals developed specific cellular responses to these proteins, although the nature of the responses varied among the animals. We demonstrated that DNA vaccination led to a CD8 mediated killing of targets expressing the homologous clade B envelope as well as targets expressing heterologous clade E envelope. In addition seronegative individuals have been inoculated with a DNA construct which expresses the env, rev proteins. These studies serve as an important benchmark for the use of DNA vaccine technology for the production of protective immune responses.
开发更有效的HIV-1疫苗的新方法具有重要意义。在本报告中，我们分析了一种DNA疫苗接种方法在黑猩猩模型系统中的免疫原性和有效性。三只黑猩猩接种了表达env、rev、gag和pol蛋白的DNA构建体。这些动物对这些蛋白产生了特异性细胞反应，尽管不同动物的反应性质有所不同。我们证明，DNA疫苗接种导致CD8介导的对表达同源B亚型包膜的靶细胞以及表达异源E亚型包膜的靶细胞的杀伤。此外，血清阴性个体接种了表达env、rev蛋白的DNA构建体。这些研究为使用DNA疫苗技术产生保护性免疫反应提供了重要的基准。
