利用单克隆抗体对小鼠冠状病毒DVIM的血凝素酯酶蛋白（HE）进行特性鉴定。
Characterization of haemagglutinin-esterase protein (HE) of murine corona virus DVIM by monoclonal antibodies.
作者信息
Kasai H, Morita E, Hatakeyama K, Sugiyama K
机构信息
Department of Biology, Faculty of Science, Hirosaki University, Japan.
出版信息
Arch Virol. 1998;143(10):1941-8. doi: 10.1007/s007050050431.
We analyzed the characteristics of seven monoclonal antibodies (mAbs) raised against purified HE (hemagglutinin-esterase) glycoprotein of the murine coronavirus DVIM (diarrhea virus of infant mice). Immunocrossreaction of these mAbs with JHM and/or MHV-S suggest that antigenic epitopes of HE of DVIM are similar to those of JHM and/or MHV-S. Four mAbs (1b4, 3a28, 4c19, 10b7), designated as group A mAbs, strongly inhibited both HA and AE activities. On the other hand, three mAbs (5a3, 6a6, 13a4), referred to as group B, had a comparatively weak HA inhibition activity. These results indicate that the antigenic epitopes of this glycoprotein can be classified into at least two groups and that the functional sites of HA and AE activities are similar but not identical. Neutralizing activity was shown in group A mAbs exclusively, suggesting that the ratio of HA and/or AE activities may play important roles in the cell fusion activity of DVIM-infected cells.
我们分析了针对鼠冠状病毒DVIM（幼鼠腹泻病毒）纯化的血凝素酯酶（HE）糖蛋白产生的七种单克隆抗体（mAb）的特性。这些mAb与JHM和/或MHV-S的免疫交叉反应表明，DVIM的HE的抗原表位与JHM和/或MHV-S的相似。四种mAb（1b4、3a28、4c19、10b7），被指定为A组mAb，强烈抑制HA和AE活性。另一方面，三种mAb（5a3、6a6、13a4），称为B组，具有相对较弱的HA抑制活性。这些结果表明，这种糖蛋白的抗原表位可至少分为两组，且HA和AE活性的功能位点相似但不完全相同。仅A组mAb显示出中和活性，表明HA和/或AE活性的比例可能在DVIM感染细胞的细胞融合活性中起重要作用。
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