Hasoksuz M, Lathrop S, Al-dubaib M A, Lewis P, Saif L J
Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, USA.
Arch Virol. 1999;144(12):2441-7. doi: 10.1007/s007050050656.
Bovine coronavirus (BCV) causes neonatal calf diarrhea (CD) and is associated with winter dysentery (WD) in adult dairy cattle. It can also be isolated from the respiratory tracts of cattle entering feedlots. Monoclonal antibodies (MAbs) specific for the hemagglutinin esterase (HE) and spike (S) surface proteins of 2 bovine enteric coronavirus (BECV) strains and two bovine respiratory coronavirus (BRCV) strains were tested against 6 BECV strains and 6 recently isolated BRCV strains, in order to characterize the antigenicity of BCV strains with varied tissue tropisms. All MAbs had high immunofluorescence (IF) titers against BECV and BRCV strains, indicative of conserved cross-reactive epitopes. In hemagglutination inhibition (HI) tests, the S-MAbs were more broadly reactive than HE-MAbs. The BRCV and CD MAbs were more broadly reactive in HI than the WD MAbs. The HA activity of the Mebus vaccine CD strain was not inhibited by any of the MAbs tested. The HI activity of BRCV strain R6 was unique among the 6 BRCV isolates. In virus neutralization assays, MAbs to the BRCV strain R4 neutralized all 6 BECV strains tested. Antigenic variation exists among both BECV and BRCV strains, but it cannot be attributed soley to the clinical origin of the strain.
牛冠状病毒（BCV）可导致新生犊牛腹泻（CD），并与成年奶牛的冬季痢疾（WD）有关。它也可从进入饲养场的牛的呼吸道中分离出来。针对2株牛肠道冠状病毒（BECV）和2株牛呼吸道冠状病毒（BRCV）的血凝素酯酶（HE）和刺突（S）表面蛋白的单克隆抗体（MAb），对6株BECV菌株和6株最近分离的BRCV菌株进行了测试，以表征具有不同组织嗜性的BCV菌株的抗原性。所有单克隆抗体对BECV和BRCV菌株均具有高免疫荧光（IF）效价，表明存在保守的交叉反应表位。在血凝抑制（HI）试验中，S单克隆抗体的反应性比HE单克隆抗体更广泛。BRCV和CD单克隆抗体在HI试验中的反应性比WD单克隆抗体更广泛。所测试的任何单克隆抗体均未抑制Mebus疫苗CD株的HA活性。BRCV菌株R6的HI活性在6株BRCV分离株中是独特的。在病毒中和试验中，针对BRCV菌株R4的单克隆抗体中和了所有测试的6株BECV菌株。BECV和BRCV菌株之间均存在抗原变异，但这不能完全归因于菌株的临床来源。
