Lakin J D, Cahill R A
J Allergy Clin Immunol. 1976 Jul;58(1 PT. 2):160-71. doi: 10.1016/0091-6749(76)90151-2.
A 6-yr-old white boy, Patient L. B., who was receiving cyclophosphamide (CY) for chemotherapy of a localized undifferentiated small cell sarcoma of the chest wall, developed generalized urticaria on three separate occasions immediately after CY administration. Serum samples were obtained 2 and 5 wk after the patient's last reaction and were examined for reaginic activity to the drug. Homocytotropic antibody activity to CY was found in the 2-wk postreaction sample by Prausnitz-Küstner type passive transfer skin testing to chimpanzees. This homocytotropic antibody was found to be of the IgE type, by means of an in vitro double-antibody coprecipitation assay measuring binding of free 14C-CY by specific immunoglobulin E and G fractions. Sera from 3 CY-treated patients who did not have adverse reactions to the drug and 6 normal, age-matched volunteers were used as control samples for the coprecipitation assay. None of the 3 CY-treated control patients had significant binding of 14C-CY by IgE when compared to normal individuals. However, it was found that all CY-treated patients had significant binding of 14C-CY by their IgG fraction when compared to normal subjects.
一名6岁白人男孩L.B.患者，因胸壁局限性未分化小细胞肉瘤接受环磷酰胺（CY）化疗，在三次单独的CY给药后立即出现全身性荨麻疹。在患者最后一次反应后的2周和5周采集血清样本，检测对该药物的反应素活性。通过对黑猩猩进行普劳斯尼茨-屈斯特纳型被动转移皮肤试验，在反应后2周的样本中发现了对CY的亲同种细胞抗体活性。通过体外双抗体共沉淀试验测量游离14C-CY与特异性免疫球蛋白E和G组分的结合，发现这种亲同种细胞抗体为IgE型。来自3名对该药物无不良反应的CY治疗患者和6名年龄匹配的正常志愿者的血清用作共沉淀试验的对照样本。与正常个体相比，3名CY治疗的对照患者中没有一人的IgE对14C-CY有显著结合。然而，发现与正常受试者相比，所有CY治疗患者的IgG组分对14C-CY有显著结合。
