Daw R, Candan S, Beck A J, Devlin A J, Brook I M, MacNeil S, Dawson R A, Short R D
Centre for Biomaterials and Tissue Engineering, Department of Engineering Materials, University of Sheffield, UK.
Biomaterials. 1998 Oct;19(19):1717-25. doi: 10.1016/s0142-9612(98)00080-5.
The purpose of this study was: (a) to examine the effect of plasma-gas composition on plasma polymer oxygen/carbon (O/C) ratio, functional group composition and stability in water, and then (b) to examine cell attachment to surfaces containing different concentrations of O/C and functional groups. Oxygen-functionalised surfaces were deposited by means of the plasma copolymerisation of acrylic acid/1,7-octadiene. The use of a diluent hydrocarbon allowed the deposition of surfaces with a range of O/C concentrations. Plasma copolymer surfaces were characterised by X-ray photoelectron spectroscopy (XPS). Changes in functional group composition with % acrylic acid monomer and the non-dispersive and dispersive parts of the surface energy of these plasma copolymers were measured. The solubility of the plasma copolymers was assessed by means of XPS. The degree of attachment of ROS 17/2.8 osteoblast-like cells to plasma copolymer surfaces deemed to be 'stable' in aqueous medium was measured. Tissue culture polystyrene (TCPS) was included as a control. Attachment was found to be greatest to the plasma copolymer surface with an O/C of 0.11. This surface had a carboxylic acid concentration of ca. 3%. Attachment did not correlate with increased surface wettability (i.e. the non-dispersive component of the surface energy).
(a) 研究等离子体-气体组成对等离子体聚合物氧/碳（O/C）比、官能团组成以及在水中稳定性的影响，然后 (b) 研究细胞对含有不同浓度O/C和官能团的表面的附着情况。通过丙烯酸/1,7-辛二烯的等离子体共聚沉积氧官能化表面。使用稀释烃可沉积具有一系列O/C浓度的表面。通过X射线光电子能谱（XPS）对等离子体共聚物表面进行表征。测量了官能团组成随丙烯酸单体百分比的变化以及这些等离子体共聚物表面能的非分散和分散部分。通过XPS评估等离子体共聚物的溶解度。测量了ROS 17/2.8成骨样细胞对在水性介质中被认为“稳定”的等离子体共聚物表面的附着程度。将组织培养聚苯乙烯（TCPS）作为对照。发现对O/C为0.11的等离子体共聚物表面的附着最大。该表面的羧酸浓度约为3%。附着与表面润湿性增加（即表面能的非分散成分）无关。
