金黄色葡萄球菌性眼内炎大鼠模型中的细胞因子表达

Cytokine expression in a rat model of Staphylococcus aureus endophthalmitis.

作者信息

Giese M J, Sumner H L, Berliner J A, Mondino B J

机构信息

Jules Stein Eye Institute and Department of Ophthalmology, University of California Los Angeles School of Medicine, 90095-7000, USA.

出版信息

Invest Ophthalmol Vis Sci. 1998 Dec;39(13):2785-90.

PMID:9856792

Abstract

PURPOSE

To examine the ability of viable Staphylococcus aureus to induce the production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, cytokine-induced neutrophil chemoattractant (CINC), and interferon (IFN)-gamma after intravitreal injection.

METHODS

Experimental rat eyes were injected with a 25-microl volume of approximately 80 colony-forming units of viable S. aureus; control eyes received sterile saline. Eyes were graded daily for signs of clinical inflammation and were removed 6, 24, 48, and 72 hours after injection. One group was prepared for histologic analysis, and vitreous was removed from the other group for cytokine analysis, using standard enzyme-linked immunosorbent assay procedures.

RESULTS

TNF-alpha, IL-1beta, CINC, and IFN-gamma were detected in experimental vitreous samples at increased levels that peaked at 24 hours. TNF-alpha, IL-1beta, and CINC declined at 48 hours, but IFN-gamma remained elevated. At 72 hours, levels returned to baseline. Statistically significant elevations of TNF-alpha, IL-1beta, and CINC were detected in experimental samples at 24, but not at 6 and 48 hours compared with levels in saline control samples (P < 0.03). A statistically significant increase in IFN-gamma was detected at 24 and 48 hours compared with control levels (P < 0.03). In experimental animals, clinical inflammation and inflammatory cells peaked at 24 hours, persisted at 48 hours, and began to decline thereafter. Neutrophils were the predominant inflammatory cell detected at 24 (72.3% of cells) and 48 (60.1%) hours. By 72 hours, the total number of inflammatory cells had decreased by 75.0%, and the cellular infiltrate had changed so that neutrophils equaled monocytes-macrophages.

CONCLUSIONS

S. aureus induced the expression of TNF-alpha, IL-1beta, CINC, and IFN-gamma. The time course of these cytokine levels could account for the clinical inflammatory responses and the entry and decline of vitreous cells in this model of bacterial endophthalmitis.

摘要

目的

研究活的金黄色葡萄球菌玻璃体内注射后诱导肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β、细胞因子诱导的中性粒细胞趋化因子（CINC）和干扰素（IFN）-γ产生的能力。

方法

向实验大鼠眼内注射25微升约含80个菌落形成单位的活金黄色葡萄球菌；对照眼注射无菌生理盐水。每天对眼睛进行临床炎症体征评分，并在注射后6、24、48和72小时摘除眼球。一组用于组织学分析，另一组的玻璃体用于细胞因子分析，采用标准酶联免疫吸附测定法。

结果

在实验玻璃体样本中检测到TNF-α、IL-1β、CINC和IFN-γ水平升高，在24小时达到峰值。TNF-α、IL-1β和CINC在48小时下降，但IFN-γ仍保持升高。72小时时，水平恢复到基线。与生理盐水对照样本相比，实验样本中TNF-α、IL-1β和CINC在24小时有统计学意义的升高，但在6和48小时没有（P<0.03）。与对照水平相比，在24和48小时检测到IFN-γ有统计学意义的增加（P<0.03）。在实验动物中，临床炎症和炎症细胞在24小时达到峰值，48小时持续存在，此后开始下降。中性粒细胞是在24小时（占细胞的72.3%）和48小时（占60.1%）检测到的主要炎症细胞。到72小时，炎症细胞总数减少了75.0%，细胞浸润发生变化，中性粒细胞与单核细胞-巨噬细胞数量相等。