Ravindranath R M, Mondino B J, Adamu S A, Pitchekian-Halabi H, Hasan S A, Glasgow B J
Jules Stein Eye Institute, University of California, Los Angeles 90024-1771, USA.
Invest Ophthalmol Vis Sci. 1995 Nov;36(12):2482-91.
To study the clinical, histopathologic, and immunologic responses to Staphylococcus aureus endophthalmitis in rats.
Experimental Lewis rats received an intravitreal injection of viable S. aureus (65 organisms), and control rats received sterile saline. The clinical scores, cellular infiltrate, delayed hypersensitivity reaction in skin tests, and serum and vitreous enzyme-linked immunosorbent assay titers of immunoglobulin (Ig) M, IgG, and IgA to ribitol teichoic acid (RTA), the major antigenic determinant of S. aureus cell wall, were measured and compared on days 3, 7, 10, 14, 21, and 30. The differences were statistically assessed using Mann-Whitney nonparametric t-tests and analysis of variance.
The red reflex was abolished in the majority of rats between days 3 and 21. Ocular inflammation resolved by day 30. The vitreous of eyes injected with S. aureus showed bacterial growth on days 3 and 7, followed by a decrease in numbers on days 10 and 14 and disappearance on days 21 and 30. In the vitreous, a peak neutrophil count was observed at day 3 that rapidly declined by day 7. The number of lymphocytes and plasma cells peaked on day 3 but declined more slowly. Plasma cells and Mott cells were seen on days 10 and 14, suggesting intraocular antibody production. IgM titers to RTA increased progressively in serum and vitreous, reached a peak on day 21, and declined on day 30. A weak IgG but absent IgA response to RTA was observed in serum and vitreous. S. aureus endophthalmitis was not associated with delayed hypersensitivity to the bacteria in skin tests.
S. aureus endophthalmitis is associated with the infiltration of neutrophils, lymphocytes, monocytes, and plasma cells in vitreous. Neutrophils, the predominant infiltrating cells, may be involved in bactericidal activity and opsonophagocytosis. In rat staphylococcal endophthalmitis, IgM rather than IgG may be the protective antibody.
研究大鼠对金黄色葡萄球菌性眼内炎的临床、组织病理学和免疫反应。
实验性Lewis大鼠接受玻璃体内注射活的金黄色葡萄球菌（65个菌），对照大鼠接受无菌盐水。在第3、7、10、14、21和30天测量并比较临床评分、细胞浸润、皮肤试验中的迟发型超敏反应以及血清和玻璃体内针对核糖醇磷壁酸（RTA，金黄色葡萄球菌细胞壁的主要抗原决定簇）的免疫球蛋白（Ig）M、IgG和IgA的酶联免疫吸附测定滴度。使用Mann-Whitney非参数t检验和方差分析对差异进行统计学评估。
在第3天至21天期间，大多数大鼠的红光反射消失。眼部炎症在第30天时消退。注射金黄色葡萄球菌的眼睛玻璃体内在第3天和第7天显示细菌生长，随后在第10天和第14天数量减少，在第21天和第30天消失。在玻璃体内，第3天观察到中性粒细胞计数峰值，到第7天迅速下降。淋巴细胞和浆细胞数量在第3天达到峰值，但下降较慢。在第10天和第14天可见浆细胞和莫特细胞，提示眼内抗体产生。血清和玻璃体内针对RTA的IgM滴度逐渐升高，在第21天达到峰值，在第30天下降。在血清和玻璃体内观察到对RTA的弱IgG反应，但无IgA反应。金黄色葡萄球菌性眼内炎与皮肤试验中对该细菌的迟发型超敏反应无关。
金黄色葡萄球菌性眼内炎与玻璃体内中性粒细胞、淋巴细胞、单核细胞和浆细胞的浸润有关。中性粒细胞作为主要浸润细胞，可能参与杀菌活性和调理吞噬作用。在大鼠葡萄球菌性眼内炎中，IgM而非IgG可能是保护性抗体。
