Sato A, Tsuji K, Yamamura M, Morita Y, Kanzaki H, Tada J, Makino H, Arata J
Department of Dermatology, Okayama University Medical School, Japan.
J Invest Dermatol. 1998 Dec;111(6):1079-84. doi: 10.1046/j.1523-1747.1998.00454.x.
Type 2 cytokines, such as interleukin-4 (IL-4) and IL-13, are associated with immunoglobulin E (IgE) production. This association has also been observed in CD8+ T cells from patients infected with leprosy and human immunodeficiency virus (HIV). Using intracellular cytokine staining and flow cytometry, the cytokine profile [IL-2, IL-4, IL-10, IL-13, and interferon (IFN)-gamma] of both CD4+ and CD8+ memory/effector T cells circulating in atopic dermatitis (AD) patients was investigated at the single cell level. The levels of type 2 cytokines in CD4+ T cells or CD8+ T cells in AD patients with high levels of serum IgE (AD-H), low levels of serum IgE (AD-L), and healthy controls were compared. Increased production of IL-4 and IL-13 in both CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells after 4 h in vitro stimulation with phorbol 12-myristate 13-acetate and ionomycin, was more prominent in AD-H patients than in AD-L patients or healthy controls, whereas IFN-gamma-producing CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells were relatively diminished in AD-H patients. CD4+ T cells and CD8 + T cells from AD-H patients, cultured for 48 h with phorbol 12-myristate 13-acetate and ionomycin, released larger amounts of IL-4 and IL-13 but smaller amounts of IFN-gamma than both types of cells from AD-L patients or healthy controls. In addition, when stimulated with immobilized anti-CD3 monoclonal antibody (MoAb) and anti-CD28 MoAb, CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells from AD-H patients contained more IL-4-producing cells but fewer IFN-gamma-producing cells compared with healthy controls. Finally, spontaneous mRNA expression of IL-4 in blood CD8+ CD45RO+ T cells isolated from AD-H patients was increased, as determined by reverse transcriptase-polymerase chain reaction. Therefore, in AD patients with high IgE levels, type 2 cytokine (IL-4 and IL-13) expression is associated with IgE production, in both CD4+ CD45RO+ T cell and CD8+ CD45RO+ T cell subsets.
2型细胞因子，如白细胞介素-4（IL-4）和IL-13，与免疫球蛋白E（IgE）的产生有关。在感染麻风病和人类免疫缺陷病毒（HIV）的患者的CD8+T细胞中也观察到了这种关联。使用细胞内细胞因子染色和流式细胞术，在单细胞水平上研究了特应性皮炎（AD）患者循环中的CD4+和CD8+记忆/效应T细胞的细胞因子谱[IL-2、IL-4、IL-10、IL-13和干扰素（IFN）-γ]。比较了血清IgE水平高（AD-H）、血清IgE水平低（AD-L）的AD患者和健康对照者的CD4+T细胞或CD8+T细胞中2型细胞因子的水平。在用佛波醇12-肉豆蔻酸酯13-乙酸酯和离子霉素体外刺激4小时后，AD-H患者的CD4+CD45RO+T细胞和CD8+CD45RO+T细胞中IL-4和IL-13的产生增加，比AD-L患者或健康对照者更明显，而产生IFN-γ的CD4+CD45RO+T细胞和CD8+CD45RO+T细胞在AD-H患者中相对减少。用佛波醇12-肉豆蔻酸酯13-乙酸酯和离子霉素培养48小时后，AD-H患者的CD4+T细胞和CD8 + T细胞释放的IL-4和IL-13量比AD-L患者或健康对照者的这两种细胞类型更多，但IFN-γ量更少。此外，当用固定化抗CD3单克隆抗体（MoAb）和抗CD28 MoAb刺激时，与健康对照相比，AD-H患者的CD4+CD45RO+T细胞和CD8+CD45RO+T细胞中产生IL-4的细胞更多，但产生IFN-γ的细胞更少。最后，通过逆转录聚合酶链反应测定，从AD-H患者分离的血液CD8+CD45RO+T细胞中IL-4的自发mRNA表达增加。因此，在IgE水平高的AD患者中，2型细胞因子（IL-4和IL-13）的表达与CD4+CD45RO+T细胞和CD
