Tuffereau C, Bénéjean J, Blondel D, Kieffer B, Flamand A
Laboratoire de Génétique des Virus, CNRS, 91198 Gif sur Yvette, France.
EMBO J. 1998 Dec 15;17(24):7250-9. doi: 10.1093/emboj/17.24.7250.
A random-primed cDNA expression library constructed from the mRNA of neuroblastoma cells (NG108) was used to clone a specific rabies virus (RV) receptor. A soluble form of the RV glycoprotein (Gs) was utilized as a ligand to detect positive cells. We identified the murine low-affinity nerve-growth factor receptor, p75NTR. BSR cells stably expressing p75NTR were able to bind Gs and G-expressing lepidopteran cells. The ability of the RV glycoprotein to bind p75NTR was dependent on the presence of a lysine and arginine in positions 330 and 333 respectively of antigenic site III, which is known to control virus penetration into motor and sensory neurons of adult mice. P75NTR-expressing BSR cells were permissive for a non-adapted fox RV isolate (street virus) and nerve growth factor (NGF) decreased this infection. In infected cells, p75NTR associates with the RV glycoprotein and could be precipitated with anti-G monoclonal antibodies. Therefore, p75NTR is a receptor for street RV.
利用从神经母细胞瘤细胞（NG108）的mRNA构建的随机引物cDNA表达文库来克隆一种特异性狂犬病病毒（RV）受体。将RV糖蛋白（Gs）的可溶性形式用作配体来检测阳性细胞。我们鉴定出了小鼠低亲和力神经生长因子受体p75NTR。稳定表达p75NTR的BSR细胞能够结合Gs以及表达G的鳞翅目细胞。RV糖蛋白结合p75NTR的能力取决于抗原位点III的第330位和第333位分别存在赖氨酸和精氨酸，已知该抗原位点III控制病毒侵入成年小鼠的运动和感觉神经元。表达p75NTR的BSR细胞对一种未适应的狐狸RV分离株（街毒）敏感，并且神经生长因子（NGF）可减少这种感染。在受感染的细胞中，p75NTR与RV糖蛋白结合，并且可以用抗G单克隆抗体沉淀。因此，p75NTR是街毒RV的一种受体。
