Pierleoni C, Verdenelli F, Castellucci M, Cinti S
Institute of Normal Human Morphology, Faculty of Medicine, University of Ancona, Italy.
Eur J Histochem. 1998;42(3):183-8.
Adipose tissue is a type of connective tissue whose extracellular matrix (ECM) components are poorly characterized in vivo. Several in vitro studies have suggested a regulatory role for the ECM molecules during adipocyte differentiation. Since no data are available concerning the in vivo expression of the main ECM components such as fibronectin, collagen IV, laminin and heparan sulfate proteoglycan in the adipose tissue, we investigated the presence of these molecules by immunohistochemistry. We show that fibronectin isoforms are not expressed in fully differentiated subcutaneous adipocytes whereas collagen IV, laminin and heparan sulfate are detectable around single adipocytes, i.e. in the region corresponding to the basement membrane. These data are supported by previous in vitro studies showing a strong decrease of fibronectin synthesis during adipocyte development whereas basement membrane molecules seem to increase during adipocyte differentiation.
脂肪组织是一种结缔组织，其细胞外基质（ECM）成分在体内的特征尚不明确。多项体外研究表明，ECM分子在脂肪细胞分化过程中具有调节作用。由于目前尚无关于脂肪组织中主要ECM成分（如纤连蛋白、IV型胶原、层粘连蛋白和硫酸乙酰肝素蛋白聚糖）体内表达的数据，我们通过免疫组织化学研究了这些分子的存在情况。我们发现，纤连蛋白异构体在完全分化的皮下脂肪细胞中不表达，而IV型胶原、层粘连蛋白和硫酸乙酰肝素在单个脂肪细胞周围（即对应于基底膜的区域）可检测到。这些数据得到了先前体外研究的支持，这些研究表明，在脂肪细胞发育过程中纤连蛋白合成显著减少，而基底膜分子在脂肪细胞分化过程中似乎增加。
