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[轮状病毒在索科乌波德拉斯基医疗保健联盟住院腹泻儿童消化道感染中的作用]

[The role of rotaviruses in digestive tract infections of hospitalized children with diarrhoea at the Health Care Consortium in Sokoł Podlaski].

作者信息

Niemińska-Gromada B, Szych J

机构信息

Pracownia Bakteriologii Zespołu Opieki Zdrowotnej w Sokołowie Podlaskim.

出版信息

Med Dosw Mikrobiol. 1998;50(1-2):77-87.

PMID:9857617
Abstract

The aim of the study was a trial of establishing of the frequency of rotavirus infection and mixed bacterial-viral infections in children treated for diarrhoea in a hospital in the period from July 1 1996 to June 30 1997. Moreover, an attempt was made at establishing of the frequency of rotavirus colonization of the digestive tract in healthy newborns born in that hospital during the period of that study. The studies were done on feces samples from 169 children with diarrhoea from 4 days to 13 years, and from 30 healthy peers. In all samples rotaviruses were sought along with pathogenic Enterobacteriaceae Tests for rotaviruses were done with Slidex-Rota Kit 2 (Bio-Merieux) and for EPEC with the EPEC latex test (Biomex). In all, rotaviruses were found in 88 ill children (52.1%) including mixed bacterial-viral infections in 10 (5.1%) children. In 9 children beside rotaviruses enteropathogenic E. coli (EPEC) were disclosed, and in 1 child S. enteritidis. Moreover, in 1 child (0.6%) culture yielded simultaneously S. sonnei and E. coli 0127. No rotaviruses were found in any 30 healthy newborns, but from one of them E. coli 018 was cultured. The second most frequent aetiological factor in diarrhoea were EPEC organisms found in 17 (10.0%) children, the third factor were S. enteritidis strains in 6 children (3.6%). In one case S. sonnei and E. coli 0127 were obtained from faces. Rotavirus infection was most frequent in children aged from 2 months to 3 years, and EPEC infection in children up to 2 years. The incidence was highest in winter/spring, with peak in April when 27 cases of rotavirus diarrhoea were noted.

摘要

该研究的目的是对1996年7月1日至1997年6月30日期间在一家医院接受腹泻治疗的儿童中轮状病毒感染及细菌 - 病毒混合感染的频率进行试验。此外,还尝试确定在该研究期间在那家医院出生的健康新生儿消化道轮状病毒定植的频率。研究对象为169名年龄从4天至13岁的腹泻儿童以及30名健康同龄人的粪便样本。在所有样本中,除了寻找轮状病毒外,还检测致病性肠杆菌科细菌。使用Slidex - Rota Kit 2(生物梅里埃公司)检测轮状病毒,使用EPEC乳胶试验(Biomex)检测肠致病性大肠杆菌(EPEC)。总共在88名患病儿童(52.1%)中发现了轮状病毒,其中10名儿童(5.1%)为细菌 - 病毒混合感染。在9名儿童中除了轮状病毒外还检测出了肠致病性大肠杆菌(EPEC),在1名儿童中检测出肠炎沙门氏菌。此外,在1名儿童(0.6%)的培养物中同时分离出宋内志贺氏菌和大肠杆菌O127。在30名健康新生儿中未发现轮状病毒,但从其中1名新生儿中培养出了大肠杆菌O18。腹泻的第二大常见病因是在17名儿童(10.0%)中发现的EPEC菌,第三大病因是在6名儿童(3.6%)中发现的肠炎沙门氏菌菌株。在1例中,从粪便中分离出宋内志贺氏菌和大肠杆菌O127。轮状病毒感染在2个月至3岁的儿童中最为常见,EPEC感染在2岁以下儿童中最为常见。发病率在冬季/春季最高,4月份达到峰值,当时记录到27例轮状病毒腹泻病例。

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