Chłap Z, Jedynak U, Sładek K
Miedzywydzialowa Katedra Patofizjologii.
Pneumonol Alergol Pol. 1998;66(5-6):321-9.
The analysis of mast cells in bronchoalveolar lavage (BAL) is rarely performed in routine diagnostic protocol. The BAL mast cells are rather difficult to be identified due to their paucity and technical problems with fixation and staining methods. The present study was carried out in order to characterize quantitatively and morphologically of mast cells obtained in bronchoalveolar lavage from 38 patients with several lung disorders. The percentage of mast cells ranged from: 0.0-0.6% (aspirin intolerant asthmatics), 0.1-1.8% (aspirin tolerant asthmatics), 0.9-1.8% (sarcoidosis), 1.5-2.0% (fibrosis), 0.04-0.08% (normals). There were statistically significant differences in the mast cells count between tested disease groups. Lung mast cells exhibited heterogeneity of size, shape, intensity of staining and degree of degranulation. Bronchoalveolar lavage is a very interesting and suitable model for the study of human lung mast cells. These cells should be analysed because of their clinical importance in pathogenesis of several lung disorders. We recommend, Carnoy's solution as a fixative and 0.5% solution of Toluidine blue (pH-0.5 with prolongation of staining time to 5-7 days at 4 degrees C) as a dye for routine diagnostic method of BAL mast cells identification.
支气管肺泡灌洗（BAL）中肥大细胞的分析在常规诊断方案中很少进行。由于支气管肺泡灌洗中的肥大细胞数量稀少，以及固定和染色方法存在技术问题，很难对其进行识别。本研究旨在对38例患有多种肺部疾病的患者支气管肺泡灌洗中获得的肥大细胞进行定量和形态学特征分析。肥大细胞的百分比范围为：0.0 - 0.6%（阿司匹林不耐受性哮喘患者），0.1 - 1.8%（阿司匹林耐受性哮喘患者），0.9 - 1.8%（结节病患者），1.5 - 2.0%（纤维化患者），0.04 - 0.08%（正常人群）。受试疾病组之间的肥大细胞计数存在统计学显著差异。肺肥大细胞在大小、形状、染色强度和脱颗粒程度方面表现出异质性。支气管肺泡灌洗是研究人肺肥大细胞的一个非常有趣且合适的模型。由于这些细胞在几种肺部疾病的发病机制中具有临床重要性，因此应该对其进行分析。我们推荐使用卡诺氏固定液作为固定剂，0.5%甲苯胺蓝溶液（pH值为0.5，在4℃下延长染色时间至5 - 7天）作为支气管肺泡灌洗肥大细胞识别常规诊断方法的染料。
