Obinata T, Hasegawa T, Masaki T, Hayashi T
J Biochem. 1976 Mar;79(3):521-31. doi: 10.1093/oxfordjournals.jbchem.a131096.
The subunit composition and immunological properties of two types of myosins, the 3S and 6S myosin components, from skeletal muscle of early chick embryos were studied by SDS-acrylamide gel electrophoresis and immunodiffusion techniques. It was shown that the 6S myosin in the early embryonic stage was composed of two heavy chains and three kinds of light chains, as is well-known in the complete myosin molecule, having the same molecular weights and the same antigenicities as corresponding subunits of the myosin from adult chicken skeletal muscle. The heavy chain of 6S myosin was also reactive with the antibody against the heavy chain of cardiac myosin. The embryonic 3S myosin was shown to be composed of a heavy chain which was roughly the same in molecular weight but not the same in antigenicity as those of adult or embryonic 6S myosin. No light chains were detected either electrophoretically or immunologically in the 3S myosin component.
利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫扩散技术，对早期鸡胚骨骼肌中两种类型的肌球蛋白（3S和6S肌球蛋白组分）的亚基组成和免疫特性进行了研究。结果表明，早期胚胎阶段的6S肌球蛋白由两条重链和三种轻链组成，这在完整的肌球蛋白分子中是众所周知的，其分子量和抗原性与成年鸡骨骼肌肌球蛋白的相应亚基相同。6S肌球蛋白的重链也与抗心肌肌球蛋白重链的抗体发生反应。胚胎期的3S肌球蛋白由一条重链组成，其分子量与成年或胚胎期6S肌球蛋白的重链大致相同，但抗原性不同。在3S肌球蛋白组分中，无论是通过电泳还是免疫方法都未检测到轻链。
