Shiotani A, Flint P W
Department of Otolaryngology and Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Neuroreport. 1998 Nov 16;9(16):3639-42. doi: 10.1097/00001756-199811160-00015.
Superfast myosin heavy chain (MHC), which is found in jaw-closing muscle and extraocular muscle (EOM), may also be found in rat laryngeal muscles. Immunostaining and Western blot using anti-EOM antibody were performed to identify and localize EOM MHC in laryngeal muscles. Specific reactivity of laryngeal IIL MHC was confirmed by Western blot and on immunostaining, all fibers in the lateral part of thyroarytenoid muscle reacted with EOM antibody. A scattered pattern of positive fibers was observed in the medial part of the thyroarytenoid, the posterior cricoarytenoid and the lateral cricoarytenoid muscles. EOM MHC was not detected in the cricothyroid muscle. The expression of EOM MHC in rat laryngeal muscle is consistent with the functional demands of the airway protection reflex.
超快速肌球蛋白重链（MHC）存在于咬肌和眼外肌（EOM）中，也可能存在于大鼠喉部肌肉中。使用抗EOM抗体进行免疫染色和蛋白质印迹，以鉴定和定位喉部肌肉中的EOM MHC。蛋白质印迹证实了喉内收肌IIL MHC的特异性反应性，并且在免疫染色中，甲杓肌外侧部分的所有纤维均与EOM抗体发生反应。在甲杓肌内侧部分、环杓后肌和环杓侧肌中观察到阳性纤维呈散在分布。在环甲肌中未检测到EOM MHC。EOM MHC在大鼠喉部肌肉中的表达与气道保护反射的功能需求一致。
