Albrecht B K, Fried B, Sherma J
Departments of Chemistry and Biology, Lafayette College, Easton, Pennsylvania 18042, USA.
J Helminthol. 1998 Dec;72(4):355-7. doi: 10.1017/s0022149x00016734.
High performance thin layer chromatography (HPTLC) was used to determine phospholipids and sphingolipids in the intestinal mucosa of ICR mice infected with Echinostoma caproni for two weeks. The major phospholipids detected in both infected and non-infected mucosa were phosphatidylcholine (PC) and phosphatidylethanolamine (PE). HPTLC-densitometric analysis showed that there was a significant decrease in the weight of both PC and PE in the intestinal mucosa of infected mice compared to that of the uninfected controls. Cerebrosides and sulphatides, but not sphingomyelin, were identified in the intestinal mucosa of both infected and uninfected hosts. There was an apparent increase in the cerebroside content of the mucosa of infected versus control mice. The pathobiochemical changes seen in the polar lipid content of infected hosts probably reflect the feeding and behavioural activities of E. caproni in the mouse intestine.
采用高效薄层色谱法（HPTLC）测定感染卡氏棘口吸虫两周的ICR小鼠肠黏膜中的磷脂和鞘脂。在感染和未感染的黏膜中检测到的主要磷脂是磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）。HPTLC光密度分析表明，与未感染的对照组相比，感染小鼠肠黏膜中PC和PE的重量均显著降低。在感染和未感染宿主的肠黏膜中均鉴定出脑苷脂和硫苷脂，但未鉴定出鞘磷脂。与对照小鼠相比，感染小鼠黏膜中的脑苷脂含量明显增加。感染宿主极性脂质含量的病理生化变化可能反映了卡氏棘口吸虫在小鼠肠道中的摄食和行为活动。
