Harnnoi T, Wijit A, Morakote N, Pipitgool V, Maleewong W
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
J Helminthol. 1998 Dec;72(4):359-61. doi: 10.1017/s0022149x00016746.
The specific gravity of the eggs of the liver fluke Opisthorchisviverrini was determined using a sucrose gradient centrifugation and found to range from 1.2713 to 1.3043. The peak egg count was located at the sucrose fraction with a specific gravity of 1.2814. An attempt to float eggs in saturated sodium nitrate solution, sp.gr. 1.4, failed. Examination of human stool specimens for Oviverrini eggs by simple flotation in saturated sodium nitrate solution and the formol-ether sedimentation technique revealed that the flotation technique was not as efficient as the sedimentation technique. It was suggested that the flotation techniques were inappropriate for the detection of Oviverrini eggs in faeces or contaminated soil.
使用蔗糖梯度离心法测定了肝吸虫猫后睾吸虫（Opisthorchis viverrini）虫卵的比重，发现其范围为1.2713至1.3043。虫卵计数峰值位于比重为1.2814的蔗糖组分处。尝试将虫卵漂浮在比重为1.4的饱和硝酸钠溶液中未成功。通过在饱和硝酸钠溶液中简单漂浮和福尔马林-乙醚沉淀技术对人体粪便标本进行猫后睾吸虫虫卵检查，结果显示漂浮技术不如沉淀技术有效。有人认为漂浮技术不适用于检测粪便或受污染土壤中的猫后睾吸虫虫卵。
