Uray N J, Gona A G
Department of Anatomy, Kirksville College of Osteopathic Medicine, Kirksville, Mo.63501, USA.
Brain Behav Evol. 1999;53(1):10-9. doi: 10.1159/000006578.
Calcium binding protein (CaBP) immunoreactivity in the cerebellum of bullfrogs was examined, concentrating on cells associated with the auricular lobe. While anti-calretinin and anti-parvalbumin also immunoreacted with the same cell populations, anti-calbindin exhibited the most robust and typical pattern of immunostaining. Calbindin immunoreactivity was observed in various populations of cells in the auricular lobe and interauricular granular band of the cerebellum, in the cerebellar peduncle, and in a bundle of interauricular commissural fibers which course through the dorsal, marginal, part of the molecular layer. Cells in the granular layer of the ventral part (i.e., corpus cerebelli) of the cerebellar plate were not CaBP-immunoreactive, nor were any fibers in the molecular layer of this cerebellar region. We believe that axons of CaBP-immunoreactive granule-like cells of the auricular lobes contribute to the formation of the interauricular fiber bundle, which corresponds to the lateral commissure of urodele amphibians. The pattern of calbindin immunoreactivity in the auricular lobes and marginal part of the cerebellar plate provides additional evidence that this cerebellar compartment, which is already present in tadpoles, has a distinct origin, biochemical characterization and connectivity and is separate from the compartment that forms the corpus cerebelli of frogs during metamorphosis.
研究了牛蛙小脑钙结合蛋白（CaBP）的免疫反应性，重点关注与耳叶相关的细胞。虽然抗钙视网膜蛋白和抗小白蛋白也与相同的细胞群体发生免疫反应，但抗钙结合蛋白表现出最强劲和典型的免疫染色模式。在小脑耳叶、小脑耳间颗粒带、小脑脚以及一束穿过分子层背侧边缘部分的耳间连合纤维中的各种细胞群体中均观察到钙结合蛋白免疫反应性。小脑板腹侧部分（即小脑体）颗粒层中的细胞以及该小脑区域分子层中的任何纤维均无CaBP免疫反应性。我们认为，耳叶中CaBP免疫反应性颗粒样细胞的轴突有助于形成耳间纤维束，该纤维束对应于有尾两栖动物的外侧连合。小脑板耳叶和边缘部分的钙结合蛋白免疫反应性模式提供了额外证据，表明这个在蝌蚪中就已存在的小脑区域有独特的起源、生化特征和连接性，并且与变态过程中形成青蛙小脑体的区域是分开的。
