Iannuzzi L, Di Meo G P, Perucatti A, Bardaro T
National Research Council (CNR), I.A.B.B.A.M., Naples (Italy).
Cytogenet Cell Genet. 1998;82(3-4):210-4. doi: 10.1159/000015102.
Commercially available human chromosome (HSA) painting probes were hybridized on river buffalo (Bubalus bubalis, 2n = 50) chromosomes by using FISH and R-banding techniques. Clear hybridization FITC-signals revealed extensive conservation of human chromosome regions in this species and demonstrated that human chromosome probes primarily paint euchromatic regions (R-bands). The present results are discussed in the light of previous gene mapping data obtained in river buffalo and ZOO-FISH data in cattle, and in relation to the standard bovine chromosome nomenclatures. In particular, HSA 8, HSA 10, HSA 11, and HSA 16+7 paint, respectively, BBU 1p, BBU 4p, BBU 5p, and BBU 24, which are homoeologous, respectively, to cattle chromosomes 25, 28, 29 and 27. Thus, these river buffalo chromosome arms can serve as markers to resolve discrepancies in the nomenclature of cattle and related species.
利用荧光原位杂交（FISH）和R显带技术，将市售的人类染色体（HSA）涂染探针与水牛（Bubalus bubalis，2n = 50）染色体进行杂交。清晰的异硫氰酸荧光素（FITC）标记杂交信号显示出该物种中人类染色体区域的广泛保守性，并表明人类染色体探针主要涂染常染色质区域（R带）。结合先前在水牛中获得的基因定位数据以及牛的荧光原位杂交（ZOO-FISH）数据，并参照标准的牛染色体命名法，对目前的结果进行了讨论。特别地，HSA 8、HSA 10、HSA 11和HSA 16 + 7分别涂染BBU 1p、BBU 4p、BBU 5p和BBU 24，它们分别与牛染色体25、28、29和27同源。因此，这些水牛染色体臂可作为标记，用于解决牛及相关物种命名中的差异。
