Sudhakar D, Fu X, Stoger E, Williams S, Spence J, Brown D P, Bharathi M, Gatehouse J A, Christou P
John Innes Centre, Colney, Norwich, United Kingdom.
Transgenic Res. 1998 Sep;7(5):371-8. doi: 10.1023/a:1008856703464.
Transgenic rice (Oryza sativa L.) plants generated through particle bombardment expressed high levels of an insecticidal protein (the snowdrop lectin, GNA) directed against sap-sucking insects. Engineered plants expressed GNA either constitutively or in a tissue specific manner, depending on the nature of the promoter used to drive expression of the gene. We used specific antibodies raised against GNA to localize its expression in phloem tissue in plants engineered with the rice sucrose synthase promoter driving GNA expression. We report here molecular, biochemical and immunological analyses for fifteen independently-derived transformants out of more than 200 plants we generated.
通过粒子轰击产生的转基因水稻（Oryza sativa L.）植株表达了高水平的针对刺吸式昆虫的杀虫蛋白（雪花莲凝集素，GNA）。根据用于驱动基因表达的启动子的性质，工程植株以组成型或组织特异性方式表达GNA。我们使用针对GNA产生的特异性抗体，将其在由驱动GNA表达的水稻蔗糖合酶启动子构建的工程植株的韧皮部组织中的表达进行定位。我们在此报告对我们所产生的200多株植株中15个独立衍生的转化体进行的分子、生化和免疫学分析。
