Kudriavtseva G A, Siusiukin A A, Khesin Ia E, Tsvetkova N E
Tsitologiia. 1976 Mar;18(3):336-41.
Comparative cytological, histological, histochemical and partially karyological studies were carried out on 8 batches of BHK-21 cell kept at different conditons. It was found that cells adapted to suspension culture differed from monolayer culture cells both morphologically and cytochemically. Suspension cells transmitted into monolayer culture displayed smaller size, changeable form, increased glycogen accumulation, decreased enzymatic activity (acid phosphatase, succinate dehydrogenase), their karyotypes tending to hypodiploidy.
对在不同条件下保存的8批BHK - 21细胞进行了比较细胞学、组织学、组织化学及部分核型分析研究。结果发现，适应悬浮培养的细胞在形态学和细胞化学方面均与单层培养细胞不同。转入单层培养的悬浮细胞呈现出较小的尺寸、可变的形态、糖原积累增加、酶活性（酸性磷酸酶、琥珀酸脱氢酶）降低，其核型倾向于亚二倍体。
