Bloch P, Simonsen P E, Weiss N, Nutman T B
Danish Bilharziasis Laboratory, Charlottenlund, Denmark.
Trans R Soc Trop Med Hyg. 1998 Sep-Oct;92(5):518-21. doi: 10.1016/s0035-9203(98)90899-9.
Infections with Dracunculus medinensis frequently occur in the same geographical area as infections with Onchocerca volvulus and Wuchereria bancrofti. This study analysed the significance of D. medinensis infections for the specificity and sensitivity of available tests for antibody-based diagnosis of onchocerciasis (using individual recombinant clones OV-10, OV-11 and OV-16, and the OV-7/OV-10/OV-16 tri-cocktail, in an enzyme-linked immunosorbent assay) and for circulating antigen-based diagnosis of bancroftian filariasis (using the TropBio and the ICT card tests). Some immunological cross-reactivity was observed with all tests. When using individual recombinant O.volvulus antigens, the highest assay indices were obtained for clone OV-10, and the lowest for clone OV-16. Testing the serum responses against the tri-cocktail of recombinant antigens did not notably improve the assay indices. Two of 40 serum samples from individuals with patent dracunculiasis gave a false positive response in the ICT test and one of these was also positive in the TropBio test. Possible implications of applying these diagnostic assays in areas endemic for dracunculiasis are discussed.
麦地那龙线虫感染经常与盘尾丝虫和班氏吴策线虫感染发生在同一地理区域。本研究分析了麦地那龙线虫感染对盘尾丝虫病基于抗体诊断的现有检测方法（在酶联免疫吸附试验中使用单个重组克隆OV - 10、OV - 11和OV - 16以及OV - 7/OV - 10/OV - 16三联鸡尾酒）的特异性和敏感性的影响，以及对班氏丝虫病基于循环抗原诊断的现有检测方法（使用TropBio和ICT卡检测）的影响。所有检测均观察到一些免疫交叉反应。使用单个重组盘尾丝虫抗原时，克隆OV - 10的检测指标最高，克隆OV - 16的检测指标最低。检测针对重组抗原三联鸡尾酒的血清反应并未显著提高检测指标。40份来自有活动性麦地那龙线虫病患者的血清样本中有两份在ICT检测中出现假阳性反应，其中一份在TropBio检测中也呈阳性。讨论了在麦地那龙线虫病流行地区应用这些诊断检测方法可能产生的影响。
