Peng Q, Wei Z, Lau B H
Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, CA 92350, USA.
Am J Chin Med. 1998;26(3-4):291-300. doi: 10.1142/S0192415X98000336.
The antioxidant effect of a Chinese medicinal herb, Fructus corni extract (FCE), was investigated using models of oxidative stress in macrophages and vascular endothelial cells. Murine macrophages (J774) were incubated with FCE at 37 degrees C and 5% CO2 for 1 hr. Oxidative burst was triggered by zymosan and measured with a fluorescent probe. FCE exhibited a concentration- dependent suppression of oxidative burst. Confluent monolayers of bovine pulmonary artery endothelial cells (PAEC) were preincubated with FCE for 20 hrs, washed, and then exposed to an organic oxidant t-butyl hydroperoxide (tBHP) for 2 hrs. Cell viability was assessed by methylthiazol tetrazolium (MTT) assay, and cell injury by the release of intracellular lactate dehydrogenase (LDH). Lipid peroxidation products of PAEC were determined by measuring thiobarbituric acid-reactive substances (TBARS). Exposure of PAEC to tBHP resulted in decreased cell viability, increased LDH release, and elevated TBARS. Preincubation of PAEC with FCE significantly reversed these changes. Our results demonstrated that FCE can protect vascular endothelial cells from oxidant injury. The data thus suggest that Fructus corni may be useful for the prevention and/or treatment of disorders associated with oxidative damage.
采用巨噬细胞和血管内皮细胞氧化应激模型，研究了中药山茱萸提取物（FCE）的抗氧化作用。将小鼠巨噬细胞（J774）与FCE在37℃、5%二氧化碳条件下孵育1小时。用酵母聚糖触发氧化爆发，并用荧光探针进行检测。FCE对氧化爆发表现出浓度依赖性抑制作用。将融合的牛肺动脉内皮细胞（PAEC）单层与FCE预孵育20小时，洗涤后，再用有机氧化剂叔丁基过氧化氢（tBHP）处理2小时。通过甲基噻唑基四氮唑（MTT）法评估细胞活力，通过细胞内乳酸脱氢酶（LDH）释放评估细胞损伤。通过测量硫代巴比妥酸反应性物质（TBARS）来测定PAEC的脂质过氧化产物。PAEC暴露于tBHP会导致细胞活力下降、LDH释放增加和TBARS升高。PAEC与FCE预孵育可显著逆转这些变化。我们的结果表明，FCE可以保护血管内皮细胞免受氧化损伤。因此，数据表明山茱萸可能对预防和/或治疗与氧化损伤相关的疾病有用。
