Yamasaki T, Lau B H
Wakunaga of America, Co., Ltd., Mission Viejo, CA 92691, USA.
Nihon Yakurigaku Zasshi. 1997 Oct;110 Suppl 1:138P-141P. doi: 10.1254/fpj.110.supplement_138.
Oxygen radical injury and lipid peroxidation have been suggested as major causes of cancer, atherosclerosis and the aging process. We examined in vitro the effect of garlic on H2O2-induced oxidant injury in bovine pulmonary artery endothelial cells (PAEC). After overnight preincubation with Aged Garlic Extract (AGE, from Wakunaga Pharmaceutical Co., Ltd., Japan) or S-allyl cysteine (SAC), PAEC monolayers were exposed to H2O2 for 3 h. Cell viability (MTT assay), lactate dehydrogenase (LDH) release, and lipid peroxidation (TBA-RS) were measured to assess oxidant injury. AGE (1-4 mg/ml) pretreatment significantly reduced the loss of cell viability induced by 50-100 microM of H2O2. AGE and SAC exhibited dose dependent inhibition of both LDH release and TBA-RS production induced by 50 microM of H2O2. The results show that AGE and SAC can protect vascular endothelial cells from oxidant injury. Numerous garlic compounds could be involved in the antioxidant properties of garlic, while there could be some prooxidant compounds derived from garlic. It is important to keep an array of antioxidant compounds to develop good herbal preparation, like AGE.
氧自由基损伤和脂质过氧化被认为是癌症、动脉粥样硬化和衰老过程的主要原因。我们在体外研究了大蒜对过氧化氢诱导的牛肺动脉内皮细胞（PAEC）氧化损伤的影响。用陈年大蒜提取物（AGE，来自日本和光制药株式会社）或S-烯丙基半胱氨酸（SAC）预孵育过夜后，将PAEC单层细胞暴露于过氧化氢中3小时。通过测量细胞活力（MTT法）、乳酸脱氢酶（LDH）释放和脂质过氧化（TBA-RS）来评估氧化损伤。AGE（1-4mg/ml）预处理显著减少了50-100μM过氧化氢诱导的细胞活力丧失。AGE和SAC对50μM过氧化氢诱导的LDH释放和TBA-RS产生均表现出剂量依赖性抑制。结果表明，AGE和SAC可以保护血管内皮细胞免受氧化损伤。许多大蒜化合物可能参与了大蒜的抗氧化特性，而大蒜中也可能存在一些促氧化化合物。开发像AGE这样良好的草药制剂时，保持一系列抗氧化化合物很重要。
