Iwasa K, Oomori Y, Tanaka H
Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Rakumo Gakuen University, Ebetsu, Japan.
Arch Histol Cytol. 1998 Oct;61(4):373-82. doi: 10.1679/aohc.61.373.
To understand the developing processes of gamma-aminobutyric acid (GABA)-containing chromaffin cells and nerve fibers in the mouse adrenal gland, we examined the tissues in various postnatal stages by immunohistochemistry using a GABA antibody. From birth until postnatal week 1, GABA-immunoreactivity was seen in very few nerve fibers, and in none of the chromaffin cells. At postnatal week 2, GABA-immunoreactivity appeared weakly in clusters of chromaffin cells and strongly in relatively numerous varicose nerve fibers. The immunoreactive nerve fibers were densely distributed in the small immunonegative chromaffin cells and large ganglion cells, but only sparsely so in the weak immunoreactive chromaffin cells. At postnatal week 3, the number of the immunoreactive chromaffin cells and nerve fibers further increased compared to that at postnatal week 2. The staining pattern of GABA-immunoreactive nerve fibers in the medullas was similar to that at postnatal week 2. From postnatal week 4 until postnatal week 8, the distribution and frequency of the immunoreactive chromaffin cells and nerve fibers were also similar to those at postnatal week 3. These results suggest that the expression of GABA in the chromaffin cells and in the nerve fibers of the mouse adrenal gland may be completed by postnatal week 3.
为了解小鼠肾上腺中含γ-氨基丁酸（GABA）的嗜铬细胞和神经纤维的发育过程，我们使用GABA抗体通过免疫组织化学方法检测了不同出生后阶段的组织。从出生到出生后第1周，在极少的神经纤维中可见GABA免疫反应性，而在嗜铬细胞中均未见到。在出生后第2周，GABA免疫反应性在嗜铬细胞簇中呈弱阳性，而在相对较多的曲张神经纤维中呈强阳性。免疫反应性神经纤维密集分布于小的免疫阴性嗜铬细胞和大的神经节细胞中，但在免疫反应性较弱的嗜铬细胞中分布稀疏。在出生后第3周，与出生后第2周相比，免疫反应性嗜铬细胞和神经纤维的数量进一步增加。髓质中GABA免疫反应性神经纤维的染色模式与出生后第2周相似。从出生后第4周到出生后第8周，免疫反应性嗜铬细胞和神经纤维的分布及频率也与出生后第3周相似。这些结果表明，小鼠肾上腺嗜铬细胞和神经纤维中GABA的表达可能在出生后第3周完成。
