Hagger R, Gharaie S, Finlayson C, Kumar D
Department of Surgery, St. George's Hospital, Tooting, London, UK.
J Auton Nerv Syst. 1998 Nov 10;73(2-3):75-9. doi: 10.1016/s0165-1838(98)00038-1.
The interstitial cells of Cajal are proposed to have a role in the control of gut motility. The aim of this study was to establish the distribution of interstitial cells of Cajal in the wall of the normal human anorectum. Interstitial cells of Cajal express the proto-oncogene c-kit. Interstitial cells of Cajal were identified in the colon by immunohistochemical staining, using a rabbit polyclonal anti-c-kit antibody. Anorectal tissue was obtained at surgical resection for carcinoma of the colorectum. Density of interstitial cells of Cajal was graded. Statistical analysis was performed using chi2 tests. In the longitudinal and circular muscle layers of the rectum interstitial cells of Cajal were seen in the bulk of the muscle layer. In the intermuscular plane interstitial cells of Cajal encased the myenteric plexus. Interstitial cells of Cajal were found at the inner margin of the circular muscle and in association with neural elements of the submuscular plexus. Within the internal anal sphincter interstitial cells of Cajal were infrequently scattered among the muscle fibres. The density of interstitial cells of Cajal in the internal anal sphincter was significantly lower than that observed in the circular muscle layer of the rectum (P = 0.014). In conclusion, interstitial cells of Cajal are evenly distributed in the layers of the muscularis propria of the rectum, but have a lower density in the internal anal sphincter.
有人提出， Cajal间质细胞在控制肠道蠕动方面发挥作用。本研究的目的是确定 Cajal间质细胞在正常人体肛门直肠壁中的分布。Cajal间质细胞表达原癌基因c-kit。使用兔多克隆抗c-kit抗体，通过免疫组织化学染色在结肠中鉴定出Cajal间质细胞。在结直肠癌手术切除时获取肛门直肠组织。对Cajal间质细胞的密度进行分级。使用卡方检验进行统计分析。在直肠的纵肌层和环肌层中，Cajal间质细胞可见于大部分肌层。在肌间平面，Cajal间质细胞包绕肌间神经丛。在环肌的内缘以及与肌下神经丛的神经成分相关处发现了Cajal间质细胞。在肛门内括约肌内，Cajal间质细胞很少散在于肌纤维之间。肛门内括约肌中Cajal间质细胞的密度明显低于直肠环肌层中的密度（P = 0.014）。总之，Cajal间质细胞均匀分布于直肠固有肌层各层，但在肛门内括约肌中的密度较低。
