Tang G, Wang J, Zhuang Z
Faculty of Public Health, Xi'an Medical University.
Zhonghua Yu Fang Yi Xue Za Zhi. 1997 Nov;31(6):334-7.
DNA damage of human leukemia (HL-60) cells caused by methyl tert-butyl ether (MTBE), a new gasoline additive, and its metabolites tert-butyl alcohol (TBA), a-hydroxyisobutyric acid (HIBA) and formaldehyde was determined by single cell gel electrophoresis (SCGE), with release of lactate dehydrogenase as an indicator for evaluating its cytotoxicity. Results showed that MTBE, TBA and HUBA at levels of 1 to 30 mmol/L could cause DNA damage in a dose-dependent pattern. Formaldehyde at level of 5 mumol/L could cause DNA damage, but at a higher level could decrease DNA migration. It suggested that MTBE and its metabolites could have genotoxicity, however, with doses causing genotoxic effects, no cytotoxic effect by MTBE, TBA and HIBA was observed, but formaldehyde presented obvious cytotoxic effect.
通过单细胞凝胶电泳（SCGE）测定了新型汽油添加剂甲基叔丁基醚（MTBE）及其代谢产物叔丁醇（TBA）、α-羟基异丁酸（HIBA）和甲醛对人白血病（HL-60）细胞的DNA损伤，并以乳酸脱氢酶的释放作为评估其细胞毒性的指标。结果表明，1至30 mmol/L的MTBE、TBA和HUBA可呈剂量依赖性地引起DNA损伤。5 μmol/L的甲醛可引起DNA损伤，但浓度较高时会减少DNA迁移。这表明MTBE及其代谢产物可能具有遗传毒性，然而，在引起遗传毒性作用的剂量下，未观察到MTBE、TBA和HIBA的细胞毒性作用，但甲醛呈现出明显的细胞毒性作用。
