抗高三尖杉酯碱的人白血病HL60细胞的特性
Characteristics of harringtonine-resistant human leukemia HL60 cell.
作者信息
He Q Y, Zhou W D, Ji L, Zhang H Q, He N G, Xue S B
机构信息
Department of Biology, Beijing Normal University, China.
出版信息
Zhongguo Yao Li Xue Bao. 1996 Sep;17(5):463-7.
AIM
To study the mechanisms of the resistance to harringtonine (Har) in the HL60 cells.
METHODS
Growth inhibition, karyotype analysis, flow cytometry, Western blotting and polymerase chain reaction.
RESULTS
The Har-resistant HL60 cell line, named HR20, showed cross resistance to homoharringtonine, doxorubicin, daunorubicin, vincristine, and colchicine. The growth doubling time and the cell numbers in G1 phase were increased. The accumulation of cellular daunorubicin in the resistant cells was obviously reduced, but distinctly increased by tetrandrine and verapamil. The numbers of telocentromeric chromosome increased and the chromosomal aberration more occured in the resistant cells. The resistant cells overexpressed multidrug resistant mdr-1 gene and P-glycoprotein 150 kDa.
CONCLUSION
The Har-resistant HL60 cell strain belonged to a multidrug resistance strain, overexpressing mdr-1 gene and P-glycoprotein.
目的
研究HL60细胞对高三尖杉酯碱（Har）耐药的机制。
方法
生长抑制、核型分析、流式细胞术、蛋白质印迹法和聚合酶链反应。
结果
对高三尖杉酯碱耐药的HL60细胞系，命名为HR20，对高三尖杉酯碱、阿霉素、柔红霉素、长春新碱和秋水仙碱表现出交叉耐药。生长倍增时间和G1期细胞数量增加。耐药细胞中柔红霉素的蓄积明显减少，但汉防己甲素和维拉帕米可使其明显增加。端着丝粒染色体数量增加，耐药细胞中染色体畸变更多见。耐药细胞中多药耐药mdr-1基因和150 kDa P-糖蛋白过度表达。
结论
对高三尖杉酯碱耐药的HL60细胞株属于多药耐药株，mdr-1基因和P-糖蛋白过度表达。
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