人早幼粒白血病HL60细胞系及其亚系中mdr-1基因表达与癌基因的相关性
Correlation between expression of mdr-1 gene and oncogenes in human promyelocytic leukemic HL60 cell line and sublines.
作者信息
Zhou W D, Zhang H Q, Fang M, Xue S B
机构信息
Department of Biology, Beijing Normal University, China.
出版信息
Zhongguo Yao Li Xue Bao. 1996 Sep;17(5):471-3.
AIM
To study the relationship between expression of oncogenes and multiple drug resistant (MDR) phenotype.
METHODS
The drug resistant level of HL60 cell line and its sublines were determined with flow cytometry. RNA Dot blot hybridization was used to identify the expression of oncogenes and mdr-1 gene.
RESULTS
The expression of mdr-1 gene was in the opposite relation with c-myc expression, but in the positive relation with c-H-ras gene expression in the multiple drug resistant cell lines. In non-MDR cell line HL60/RA, the expression levels of mdr-1, c-myc, and c-H-ras were the same as HL60 parental cells.
CONCLUSION
Multiple drug resistance is related to not only mdr-1 expression, but also some oncogenes expression level.
目的
研究癌基因表达与多药耐药（MDR）表型之间的关系。
方法
采用流式细胞术检测HL60细胞系及其亚系的耐药水平。运用RNA斑点杂交技术鉴定癌基因和mdr - 1基因的表达。
结果
在多药耐药细胞系中，mdr - 1基因的表达与c - myc表达呈负相关，但与c - H - ras基因表达呈正相关。在非MDR细胞系HL60/RA中，mdr - 1、c - myc和c - H - ras的表达水平与HL60亲代细胞相同。
结论
多药耐药不仅与mdr - 1表达有关，还与某些癌基因的表达水平有关。
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