English R S, Williams C A, Lorbach S C, Shively J M
Department of Biological Sciences, Clemson University, SC 29634.
FEMS Microbiol Lett. 1992 Jul 1;73(1-2):111-9. doi: 10.1016/0378-1097(92)90593-d.
The autotrophic, sulfur-oxidizing bacterium Thiobacillus denitrificans possesses two forms of the Calvin cycle enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). The form I and form II genes were isolated from a cosmid library using heterologous DNA probes. Restriction enzyme analysis indicated that the genes are within 17 kbp of each other. Other Calvin cycle enzyme genes are not present. Analysis of T. denitrificans RNA indicated that the form I genes for the large and small subunits are co-transcribed with a length of 2800 nucleotides. The transcript for the form II gene is 1900 nucleotides in length.
自养型硫氧化细菌反硝化硫杆菌拥有两种形式的卡尔文循环酶核酮糖-1,5-二磷酸羧化酶/加氧酶（RuBisCO）。利用异源DNA探针从黏粒文库中分离出了I型和II型基因。限制性内切酶分析表明，这些基因彼此相距在17千碱基对以内。不存在其他卡尔文循环酶基因。对反硝化硫杆菌RNA的分析表明，I型基因的大亚基和小亚基共同转录，长度为2800个核苷酸。II型基因的转录本长度为1900个核苷酸。
