[Selective quantitative determination of single endogenously liberated nonesterified fatty acids in the blood of surgical patients].

Under defined external conditions of metabolism, a selective and quantitative determination of the single nonesterified fatty acids (NEFA) in serum allows the characterization of the interrelationship between the adipose tissue as a deposit of substrates for longterm energy production and the energy consumpting cells on the one side, as also the estimation of the extent of the stress itself. Compared with the amount of the whole lipidfraction, the very low concentrated NEFA may indicate the status of the endogenous delivery of energy-substrates only when analyzed with a high efficient selective, quantitative and direct method. The main question of the presented investigations on 14 surgical patients was the influence of an acute event of aggression on quality and quantity of fatty acid mobilization. After detailed characterization of experimental methodological conditions, the concentration-profiles and amounts of the mobilized NEFA and their modifications by the stress-reaction are reported.