油酰雌酮不会改变正常体型和肥胖型 Zucker 大鼠下丘脑神经肽 Y 的水平。

Oleoyl-estrone does not alter hypothalamic neuropeptide Y in Zucker lean and obese rats.

作者信息

Cabot C, Grasa M M, Adán C, Pérez-Clausell J, Virgili J, Estruch J, Fernández-Löpez J A, Remesar X, Alemany M

机构信息

Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Spain.

出版信息

Peptides. 1998;19(9):1631-5. doi: 10.1016/s0196-9781(98)00104-1.

DOI:10.1016/s0196-9781(98)00104-1

PMID:9864070

Abstract

Female Zucker lean and obese rats were treated for 14 days with 3.5 micromol/kg oleoyl-estrone (OE) in liposomes (Merlin-2). After 0, 3, 6, 10, and 14 days of treatment, the rats were killed and hypothalamic nuclei (lateral preoptic, median preoptic, paraventricular, ventromedial and arcuate) were used for neuropeptide Y (NPY) radioimmunoassay. In 14 days, OE decreased food intake by 26% in lean and 38% in obese rats and energy expenditure by 6% in lean and 47% in obese rats; the body weight gap between controls and treated rats becoming -17.8% of initial b.wt. in the lean and -13.6% in the obese rats. Obese rats showed higher NPY levels in all the nuclei than the lean rats. Despite a negative energy balance and decreased food intake, there were practically no changes in NPY with OE treatment. The results indicate that oleoyl-estrone does not act through NPY in its control of either food intake or thermogenesis in lean and genetically obese rats.

摘要

雌性Zucker瘦型和肥胖型大鼠用脂质体（Merlin - 2）中的3.5微摩尔/千克油酰雌酮（OE）处理14天。在处理0、3、6、10和14天后，处死大鼠，取下丘脑核（外侧视前核、正中视前核、室旁核、腹内侧核和弓状核）用于神经肽Y（NPY）放射免疫测定。在14天内，OE使瘦型大鼠的食物摄入量减少26%，肥胖型大鼠减少38%；使瘦型大鼠的能量消耗减少6%，肥胖型大鼠减少47%；对照组和处理组大鼠之间的体重差距在瘦型大鼠中变为初始体重的-17.8%，在肥胖型大鼠中变为-13.6%。肥胖大鼠所有核中的NPY水平均高于瘦型大鼠。尽管能量平衡为负且食物摄入量减少，但OE处理后NPY几乎没有变化。结果表明，在瘦型和遗传性肥胖型大鼠中，油酰雌酮在控制食物摄入或产热方面并非通过NPY起作用。