Tsuda H, Iwase T, Matsumoto K, Ito M, Hirono I, Nishida Y, Takasuka N, Iwahori Y, Ota T, Kim D J, Kadenbach B
Experimental Pathology and Chemotherapy Division, National Cancer Center Research Institute, Tokyo, Japan.
Toxicol Pathol. 1998 Nov-Dec;26(6):769-76. doi: 10.1177/019262339802600609.
The histogenesis of 3 types of rat renal cell tumors (basophilic cell, clear cell, and oncocytic) was stereologically analyzed, with particular attention paid to transitions from normal tubules. Early nitrosamine-induced preneoplastic lesions, including dysplastic tubules (altered tubules), epithelial hyperplasias, and small adenomas, were reconstructed using serially sectioned specimens processed for carbonic anhydrase type II (CA) and periodic acid-Schiff (PAS) (CA-PAS) double staining to allow easier distinction of the nephron segments: Proximal tubules had a PAS-positive brush border and were weakly positive for CA in the cytoplasm; distal tubules were PAS negative and weakly positive for CA; collecting ducts were PAS negative and strongly positive for CA. Similarly, cytochrome c oxidase (CytOx) and CytOx-PAS double staining was also applied to confirm the character of oncocytic lesions. All basophilic lesions (7 of 7) showed transition to proximal tubules. Clear cell lesions positive for CA, on the other hand, showed transition to distal tubules in 4 of 9 (44.4%) lesions and to collecting ducts in 4 of 9 (44.4%) lesions, but in only 1 of 9 (11%) to a proximal tubule. All oncocytic lesions (16 of 16), characterized by positivity for both CA and CytOx, showed transition to collecting ducts. The results indicate that the origins of renal cell neoplasia are proximal tubules for the basophilic cell lesions, either proximal or distal tubules for their clear cell counterparts, and collecting ducts for oncocytic lesions.
对3种类型的大鼠肾细胞肿瘤（嗜碱性细胞、透明细胞和嗜酸性细胞）的组织发生进行了体视学分析，特别关注了从正常肾小管的转变。使用经碳酸酐酶II型（CA）和过碘酸希夫（PAS）（CA-PAS）双重染色处理的连续切片标本，重建早期亚硝胺诱导的癌前病变，包括发育异常的肾小管（改变的肾小管）、上皮增生和小腺瘤，以便更轻松地区分肾单位节段：近端肾小管有PAS阳性的刷状缘，细胞质中CA呈弱阳性；远端肾小管PAS阴性，CA呈弱阳性；集合管PAS阴性，CA呈强阳性。同样，细胞色素c氧化酶（CytOx）和CytOx-PAS双重染色也用于确认嗜酸性病变的特征。所有嗜碱性病变（7例中的7例）均显示向近端肾小管转变。另一方面，CA阳性的透明细胞病变中，9例中有4例（44.4%）显示向远端肾小管转变，9例中有4例（44.4%）显示向集合管转变，但只有9例中的1例（11%）显示向近端肾小管转变。所有嗜酸性病变（16例中的16例），其特征为CA和CytOx均呈阳性，均显示向集合管转变。结果表明，肾细胞肿瘤的起源对于嗜碱性细胞病变是近端肾小管，对于其透明细胞对应物是近端或远端肾小管，对于嗜酸性细胞病变是集合管。
