Sato K, Kawasaki H, Nagayama H, Serizawa R, Ikeda J, Morimoto C, Yasunaga K, Yamaji N, Tadokoro K, Juji T, Takahashi T A
Department of Cell Processing, Department of Clinical Immunology and AIDS Research Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Blood. 1999 Jan 1;93(1):34-42.
We examined the potential involvement of two CC chemokine receptors (CCRs), CCR-1 and CCR-3, in the functional activation of granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interleukin-4 (IL-4)-generated human peripheral blood monocyte-derived immature dendritic cells (DCs). Flow cytometric analysis showed that CCR-1, CCR-3, CCR-5, and CXC chemokine receptor (CXCR)-4 were expressed on the cell surface of monocyte-derived DCs. Treatment with a monoclonal antibody (MoAb) to either CCR-1 or CCR-3 but not MoAbs to CCR-5 and CXCR-4 abolished chemotactic migration of monocyte-derived DCs. The DCs treated with either the anti-CCR-1 MoAb or anti-CCR-3 MoAb were less efficient than untreated DCs in proliferation of allogeneic T cells (TCs) and TC-derived secretion of interferon-gamma (IFN-gamma). The homotypic aggregation of DCs and heterotypic aggregation of DCs with TCs were suppressed by the anti-CCR-1 MoAb or anti-CCR-3 MoAb. These results indicate that CCR-1 and CCR-3 specifically regulate interaction of TCs and DCs in the process of antigen presentation.
我们研究了两种CC趋化因子受体（CCR），即CCR-1和CCR-3，在粒细胞-巨噬细胞集落刺激因子（GM-CSF）加白细胞介素-4（IL-4）诱导产生的人外周血单核细胞来源的未成熟树突状细胞（DC）功能激活中的潜在作用。流式细胞术分析显示，CCR-1、CCR-3、CCR-5和CXC趋化因子受体（CXCR）-4在单核细胞来源的DC细胞表面表达。用针对CCR-1或CCR-3的单克隆抗体（MoAb）处理单核细胞来源的DC可消除其趋化迁移，而用针对CCR-5和CXCR-4的MoAb处理则无此作用。用抗CCR-1 MoAb或抗CCR-3 MoAb处理的DC在同种异体T细胞（TC）增殖及TC分泌干扰素-γ（IFN-γ）方面的效率低于未处理的DC。抗CCR-1 MoAb或抗CCR-3 MoAb可抑制DC的同型聚集以及DC与TC的异型聚集。这些结果表明，CCR-1和CCR-3在抗原呈递过程中特异性调节TC与DC的相互作用。
