半胱氨酸蛋白酶表达的反义抑制作用影响溶组织内阿米巴诱导的仓鼠肝脓肿形成。
Antisense inhibition of expression of cysteine proteinases affects Entamoeba histolytica-induced formation of liver abscess in hamsters.
作者信息
Ankri S, Stolarsky T, Bracha R, Padilla-Vaca F, Mirelman D
机构信息
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel.
出版信息
Infect Immun. 1999 Jan;67(1):421-2. doi: 10.1128/IAI.67.1.421-422.1999.
Abstract
Trophozoites of virulent Entamoeba histolytica transfected with the antisense gene encoding cysteine proteinase 5 (CP5) have only 10% of the CP activity but retain their cytopathic activity on mammalian monolayers. In the present study we found that the transfected trophozoites with low levels of CP activity were incapable of inducing the formation of liver lesions in hamsters.
摘要
用编码半胱氨酸蛋白酶5（CP5）的反义基因转染的强毒溶组织内阿米巴滋养体仅具有10%的CP活性，但在哺乳动物单层细胞上仍保留其细胞病变活性。在本研究中，我们发现CP活性水平低的转染滋养体不能在仓鼠中诱导肝损伤的形成。
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