Ankri S, Stolarsky T, Mirelman D
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel.
Mol Microbiol. 1998 May;28(4):777-85. doi: 10.1046/j.1365-2958.1998.00837.x.
Inhibition of most of the expression of the cysteine proteinases of Entamoeba histolytica strain HM-1:IMSS was successfully performed by transcription of ehcp5 antisense RNA using the promoter of ehg34, which encodes a L21 ribosomal protein of E. histolytica. We have generated a stable transfectant in which the overall level of cysteine proteinase activity is strongly reduced ( 90%). This transfectant has a normal growth rate in Diamond's TYI-S-33 medium, a cytopathic and haemolytic activity similar to the control HM-1:IMSS pEhAct-Neo transfectant but with a significantly lower phagocytic activity.
利用编码溶组织内阿米巴L21核糖体蛋白的ehg34启动子转录ehcp5反义RNA，成功抑制了溶组织内阿米巴菌株HM-1:IMSS中大多数半胱氨酸蛋白酶的表达。我们构建了一个稳定转染子，其中半胱氨酸蛋白酶活性的总体水平大幅降低（90%）。该转染子在戴蒙德氏TYI-S-33培养基中的生长速率正常，其细胞病变和溶血活性与对照HM-1:IMSS pEhAct-Neo转染子相似，但吞噬活性显著较低。
