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主要血红蛋白衍生物的分光光度法定量分析。

Spectrophotometric quantitative analysis of the main hemoglobin derivatives.

作者信息

Adamov S A, Aleksandrova S A, Denisov A N, Mosur E Y, Semikolenova N A

机构信息

Sensor Microelectronics Institute, Siberian Branch of the Russian Academy of Sciences, Omsk, 644077, Russia.

出版信息

Biochemistry (Mosc). 1998 Oct;63(10):1160-3.

PMID:9864449
Abstract

A spectrophotometric multiwavelength method for the simultaneous determination of the main hemoglobin derivatives including oxyhemoglobin, deoxyhemoglobin, carboxyhemoglobin, and methemoglobin has been developed. The choice of analytical wavelengths was made by the methods of information factors and linear programming. The software for the quantitative analysis was developed considering the least square method, linear programming, algebraic background correction, and combined methods including linear programming and algebraic background correction.

摘要

已开发出一种用于同时测定主要血红蛋白衍生物(包括氧合血红蛋白、脱氧血红蛋白、碳氧血红蛋白和高铁血红蛋白)的分光光度多波长方法。通过信息因子法和线性规划法选择分析波长。定量分析软件的开发考虑了最小二乘法、线性规划、代数背景校正以及包括线性规划和代数背景校正在内的组合方法。

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