Senger P L, Saacke R G
J Reprod Fertil. 1976 Jul;47(2):215-9. doi: 10.1530/jrf.0.0470215.
Addition of cow serum to diluted bull spermatozoa induced marked head-to-head agglutination which declined during incubation for 9 hr at 37 degrees C. Maintenance of the acrosomal cap during incubation was not adversely affected by serum. Agglutinating cells were those with intact acrosomal and cell membranes, as determined by differential interference-contrast and electron microscopy. Single cells in the same treatment groups experienced more rapid acrosomal deterioration than did untreated spermatozoa. Ultrastructurally, agglutinated cells were associated by a close apposition of the cell membranes in the acrosomal region. Structural integrity of the cell membrane and acrosome of agglutinated cells was the same after 9 hr of incubation as at the onset of the incubation period.
向稀释的公牛精子中添加牛血清会诱导明显的头对头凝集，这种凝集在37℃孵育9小时期间会下降。孵育期间顶体帽的维持未受到血清的不利影响。通过微分干涉相差显微镜和电子显微镜确定，发生凝集的细胞是那些顶体和细胞膜完整的细胞。相同处理组中的单个细胞比未处理的精子经历了更快的顶体退化。在超微结构上，凝集的细胞通过顶体区域细胞膜的紧密并列而相互关联。孵育9小时后，凝集细胞的细胞膜和顶体的结构完整性与孵育开始时相同。
