Nagae T, Yanagimachi R, Srivastava P N, Yanagimachi H
Fertil Steril. 1986 May;45(5):701-7. doi: 10.1016/s0015-0282(16)49344-1.
Human spermatozoa were allowed to undergo the acrosome reaction in vitro by incubation in media with or without reagents known to accelerate the onset of the acrosome reaction. The first observable change before the acrosome reaction was a partial decondensation of the acrosomal matrix. This was followed by invaginations of the outer acrosomal membrane alone or of both the plasma and outer acrosomal membranes, which resulted in formation of many vesicles within the acrosomal cap. Subsequently, the plasma and outer acrosomal membranes fused, but the fusion was seldom seen in the acrosomal cap region. On the other hand, fusion of the two membranes was observed consistently at the anterior end of the equatorial segment of the acrosome. Soon after the membranes over the acrosomal cap disappeared, many vesicles, which were originally within the cap, were seen on or in the vicinity of the inner acrosomal membrane. These vesicles dispersed eventually, leaving the inner acrosomal membrane completely exposed. Thus the acrosome reaction in human spermatozoa seems to proceed in a way somewhat different from that in spermatozoa of most other species, although the end result of the reaction is the same.
将人类精子置于含有或不含有已知可加速顶体反应起始的试剂的培养基中进行体外孵育，使其发生顶体反应。顶体反应之前首先可观察到的变化是顶体基质的部分解聚。随后，仅顶体外膜或质膜和顶体外膜都会内陷，导致顶体帽内形成许多小泡。随后，质膜和顶体外膜融合，但在顶体帽区域很少见到这种融合。另一方面，在顶体赤道段前端始终观察到这两层膜的融合。顶体帽上方的膜消失后不久，原本在顶体内的许多小泡出现在顶体内膜上或其附近。这些小泡最终分散，使顶体内膜完全暴露。因此，人类精子的顶体反应似乎以某种与大多数其他物种精子不同的方式进行，尽管反应的最终结果是相同的。
