Stein G I, Panteleev V G, Povarkova A V, Kudriavtsev B N
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Tsitologiia. 1998;40(10):913-6.
Precision features of image analyser "Videotest" and possibilities of its applications for cytophotometric investigations have been analysed. It has been shown that a photometric feature is linear at least to optical density 1.1. Cytophotometry of the Feulgen stained rat hepatocyte nuclei has been performed. Coefficients of variation of DNA for 2c, 4c and 8c nuclei are 7.72, 7.65 and 6.50%, respectively, and ratios between average values of these nuclei are 1.00:2.06:4.12. Possible causes of errors of digital television equipment for cytophotometry are discussed.
分析了图像分析仪“Videotest”的精确特性及其在细胞光度测定研究中的应用可能性。结果表明，光度特性至少在光密度为1.1时呈线性。对福尔根染色的大鼠肝细胞核进行了细胞光度测定。2c、4c和8c细胞核DNA的变异系数分别为7.72%、7.65%和6.50%，这些细胞核平均值之间的比率为1.00:2.06:4.12。讨论了细胞光度测定用数字电视设备误差的可能原因。
