Henry M D, Campbell K P
Howard Hughes Medical Institute, Department of Physiology and Biophysics, The University of Iowa College of Medicine, Iowa City 52242, USA.
Cell. 1998 Dec 11;95(6):859-70. doi: 10.1016/s0092-8674(00)81708-0.
Basement membranes are composed of ordered arrays of characteristic extracellular matrix proteins, but little is known about the assembly of these structures in vivo. We have investigated the function of dystroglycan, a cell-surface laminin receptor expressed by cells contacting basement membranes in developing and adult tissues. We find that dystroglycan is required for the formation of a basement membrane in embryoid bodies. Our results further indicate that dystroglycanlaminin interactions are prerequisite for the deposition of other basement membrane proteins. Dystroglycan may exert its influence on basement membrane assembly by binding soluble laminin and organizing it on the cell surface. These data establish a role for dystroglycan in the assembly of basement membranes and suggest fundamental mechanisms underlying this process.
基底膜由特征性细胞外基质蛋白的有序阵列组成，但对于这些结构在体内的组装了解甚少。我们研究了营养不良聚糖的功能，它是一种细胞表面层粘连蛋白受体，在发育中和成年组织中与基底膜接触的细胞表达。我们发现，胚胎体中基底膜的形成需要营养不良聚糖。我们的结果进一步表明，营养不良聚糖与层粘连蛋白的相互作用是其他基底膜蛋白沉积的先决条件。营养不良聚糖可能通过结合可溶性层粘连蛋白并将其在细胞表面进行组织，从而对基底膜组装产生影响。这些数据确立了营养不良聚糖在基底膜组装中的作用，并提示了这一过程的基本机制。
