Retz M, Lehmann J, Röder C, Plötz B, Harder J, Eggers J, Pauluschke J, Kalthoff H, Stöckle M
Department of Urology, University Hospital Kiel, Germany.
Cancer Res. 1998 Dec 15;58(24):5662-6.
Mucins (MUCs) are high molecular weight membrane glycoproteins. The gene expression of MUCs (MUC1-MUC8) may change characteristically during malignant transformation of epithelial tissues. Total RNA was isolated from the four bladder cancer cell lines RT4, 647V, HT1376, and 486P (pathological gradings between G1 and G4) and 17 samples of transitional cell carcinomas, as well as 16 samples of normal human urothelium of the bladder from surgically removed specimens. The RNA samples were studied with MUC1-, MUC2- and MUC7-specific nested reverse transcription-PCRs. Gene expression of MUC1 and MUC2 was found positive in all normal, as well as in malignant, tissue samples and in the tumor cell lines. In contrast, gene expression of MUC7 was only detected in bladder cancer cell lines and samples of invasive transitional cell carcinomas, but neither in superficial, noninvasive bladder tumors nor normal bladder urothelium. Only one of the samples of normal urothelium obtained from 16 different tumor-bearing bladders was positive for MUC7 gene expression. These results suggest a differential MUC7 gene expression with the onset of malignant transformation of the bladder urothelium.
黏蛋白（MUCs）是高分子量的膜糖蛋白。在上皮组织恶性转化过程中，MUCs（MUC1 - MUC8）的基因表达可能会发生特征性变化。从四种膀胱癌细胞系RT4、647V、HT1376和486P（病理分级在G1和G4之间）以及17份移行细胞癌样本和16份取自手术切除标本的正常人膀胱尿路上皮样本中分离出总RNA。用MUC1、MUC2和MUC7特异性巢式逆转录 - PCR对RNA样本进行研究。发现MUC1和MUC2的基因表达在所有正常组织、恶性组织样本以及肿瘤细胞系中均呈阳性。相比之下，MUC7的基因表达仅在膀胱癌细胞系和浸润性移行细胞癌样本中检测到，而在浅表性、非浸润性膀胱肿瘤和正常膀胱尿路上皮中均未检测到。从16个不同的带瘤膀胱获取的正常尿路上皮样本中只有1份MUC7基因表达呈阳性。这些结果表明，随着膀胱尿路上皮恶性转化的开始，MUC7基因表达存在差异。
