Howell D N, Payne C M, Miller S E, Shelburne J D
Department of Pathology, Veterans Affairs Medical Center, Durham, NC 27705, USA.
Hum Pathol. 1998 Dec;29(12):1339-46. doi: 10.1016/s0046-8177(98)90001-6.
The power of electron microscopy as a diagnostic tool can be amplified considerably by the application of ancillary preparative and analytic methods. Subcellular chemistry and structure can be examined by various forms of microprobe analysis and by special staining methods, including cytochemical, immunocytochemical, and negative staining. Qualitative ultrastructural examination can be augmented by morphometric analysis. Correlative microscopic survey methods can be used as a means of targeting ultrastructural investigations. This article provides an overview of the use of these special techniques in the diagnosis and classification of tumors and other selected pathologic processes.
通过应用辅助制备和分析方法，电子显微镜作为一种诊断工具的能力可以得到显著增强。亚细胞化学和结构可以通过各种形式的微探针分析以及特殊染色方法进行检查，包括细胞化学、免疫细胞化学和负染色。定性超微结构检查可以通过形态计量分析得到加强。相关显微镜检查方法可以用作靶向超微结构研究的手段。本文概述了这些特殊技术在肿瘤及其他特定病理过程的诊断和分类中的应用。
