Mori N, Yatabe Y, Oka K, Kinoshita T, Kobayashi T, Ono T, Asai J
First Department of Pathology, Nagoya University School of Medicine, Japan.
Am J Pathol. 1996 Aug;149(2):699-705.
Eight patients with nasal lymphoma in whom fresh-frozen tissues were available were studied to elucidate the nature of the lymphoma cells. Two cases were diagnosed as diffuse, large cell lymphoma, and the remaining six cases as diffuse, mixed cell types. Immunohistochemical studies revealed that all of the cases were positive for perforin, which is a specific marker for cytotoxic T or natural killer (NK) cells. As all of the cases were CD8 negative, the perforin-positive finding further confirmed the concept that nasal lymphoma is a distinct neoplastic entity derived from NK or NK-related cells. Light microscopic immunohistochemical studies revealed that these nasal lymphoma cases could be classified into Leu19(CD56)+Leu4(CD3)+ (two cases) and Leu19(CD56)+Leu4(CD3)- (six cases) types according to the phenotypes of the proliferating cells. However, simultaneous staining for perforin and Leu4 (CD3) using immunoelectron microscopy on the Leu19+Leu4+ cases showed that the perforin-positive cells were different from the Leu4-positive cells. This finding suggests that the Leu4-positive cells are not neoplastic NK cells but reactive T cells. Six cases were positive for EBER-1 by in situ hybridization analysis. This finding reconfirms the previous studies that Epstein-Barr virus plays a significant role in the pathogenesis of nasal lymphoma.
对8例有新鲜冷冻组织的鼻腔淋巴瘤患者进行研究，以阐明淋巴瘤细胞的性质。2例被诊断为弥漫性大细胞淋巴瘤，其余6例为弥漫性混合细胞类型。免疫组织化学研究显示，所有病例的穿孔素均呈阳性，穿孔素是细胞毒性T细胞或自然杀伤（NK）细胞的特异性标志物。由于所有病例的CD8均为阴性，穿孔素阳性结果进一步证实了鼻腔淋巴瘤是一种源自NK或NK相关细胞的独特肿瘤实体的概念。光镜免疫组织化学研究显示，根据增殖细胞的表型，这些鼻腔淋巴瘤病例可分为Leu19（CD56）+Leu4（CD3）+（2例）和Leu19（CD56）+Leu4（CD3）-（6例）类型。然而，对Leu19+Leu4+病例使用免疫电子显微镜同时对穿孔素和Leu4（CD3）进行染色显示，穿孔素阳性细胞与Leu4阳性细胞不同。这一发现表明，Leu4阳性细胞不是肿瘤性NK细胞，而是反应性T细胞。6例原位杂交分析EBER-1呈阳性。这一发现再次证实了先前的研究，即爱泼斯坦-巴尔病毒在鼻腔淋巴瘤的发病机制中起重要作用。
