Paster I P, Tron'ko M D
V. P. Komisarenko Institute of Endocrinology and Metabolism, AMS, Kiev.
Fiziol Zh (1994). 1998;44(5-6):21-6.
In experiments with a primary culture of adrenal cells, it has been studied the influence of chloditane, as a specific inhibitor of adrenocortical function, on the synthetic processes in the newborn pig adrenal cells, whose organ and cell cultures are more and more used as transplantation material for the treatment of adrenal insufficiency. It has been shown that incubation of adrenocorticocytes with chloditane (final concentration in culture medium: 1 to 10,000 ng/ml) during 24 hours had a marked negative dose-dependent effect on the indices of inclusion of 3H-thymidine and 3H-uridine into the nucleic acids. In the presence of a maximum chloditane concentration, inclusion made, respectively, 16.9 and 46.0% from control. A trustworthy effect of chloditane on the indices of 3H-leucine inclusion (protein synthesis) has been established only for concentration 10 to 100 ng/ml and made near 70% from control. It has been discussed a possible mechanism of chloditane action on the synthetic processes in adrenal cells.
在肾上腺细胞原代培养实验中，研究了作为肾上腺皮质功能特异性抑制剂的氯地孕酮对新生猪肾上腺细胞合成过程的影响，猪肾上腺的器官和细胞培养物越来越多地被用作治疗肾上腺功能不全的移植材料。结果表明，肾上腺皮质细胞与氯地孕酮（培养基中的终浓度：1至10,000 ng/ml）一起孵育24小时，对3H-胸腺嘧啶核苷和3H-尿苷掺入核酸的指标有明显的负剂量依赖性作用。在氯地孕酮最大浓度存在时，掺入量分别为对照的16.9%和46.0%。仅在浓度为10至100 ng/ml时，氯地孕酮对3H-亮氨酸掺入指标（蛋白质合成）有可靠作用，且为对照的近70%。文中讨论了氯地孕酮对肾上腺细胞合成过程作用的可能机制。
